Citation: Wen-Ge Ma, Su-Qin Lu, De-Bin Zhu, Xiao-Bo Xing, Bi-Hua Su. Isothermal detection of RNA transcription levels using graphene oxide-based SYBR Green I fluorescence platform[J]. Chinese Chemical Letters, ;2014, 25(3): 482-484. doi: 10.1016/j.cclet.2013.12.013 shu

Isothermal detection of RNA transcription levels using graphene oxide-based SYBR Green I fluorescence platform

Wen-Ge Ma ^a,, ,
Su-Qin Lu ^b ,
De-Bin Zhu ^c ,
Xiao-Bo Xing ^c ,
Bi-Hua Su ^d

1.
a Guangzhou Nansha Center for Disease Control & Prevention, Guangzhou 511457, China;
2.
b Department of Medical Ultrasonics, The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510630, China;
3.
c MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China;
4.
d Guangzhou Baiyun District Center for Disease Control & Prevention, Guangzhou 510445, China

Corresponding author: Wen-Ge Ma,
Received Date: 4 November 2013
Available Online: 12 December 2013
Fund Project: This research is supported by the National Natural Science Foundation of China (Nos. 81071790, 81371877, 61177077) (Nos. 81071790, 81371877, 61177077) the Key Project of Chinese Ministry of Education (No. 211131) (No. 211131) and the Postdoctoral Foundation of China (No. 201003359). (No. RG201001003)

An isothermal graphene oxide (GO)-based SYBR Green I fluorescence platform for sensitive detection of RNA transcription levels is proposed. Briefly, a synthesized T7 DNA template was transcribed by T7 RNA polymerase, to producemany copies of single-stranded (ss) RNA transcripts. The ssRNA transcripts were then hybridized with a label-free ssDNA probe which alone will be adsorbed on the GO surface. The resultant double-stranded (ds) RNA:DNA hybrids were incubated with GO and SYBR Green I. Then the hybrid binding with SYBR Green I emits enhanced fluorescence for detection. Experimental results show that the detection limit of the method is 0.5 pmol/L of T7 DNA template. A calibration curve with a linearity range of 0.5 pmol/L to 5 nmol/L is established, thus, making quantitative analysis feasible. The method may become a powerful tool for RNA transcription detection due to its sensitivity, rapidity and convenience.
- Isothermal detection,
- RNA transcription level,
- Graphene oxide,
- SYBR Green I,
- Fluorescence
1. [1]
  [1] W.J. Blake, M. Kaern, C.R. Cantor, J.J. Collins, Noise in eukaryotic gene expression, Nature 422 (2003) 633-637.
2. [2]
  [2] K. Lü, G.X. Zhao, X.K. Wang, A brief review of graphene-based material synthesis and its application in environmental pollution management, Chin. Sci. Bull. 57 (2012) 1223-1234.
3. [3]
  [3] Z.B. Liu, X.L. Zhang, X.Q. Yan, Y.S. Chen, J.G. Tian, Nonlinear optical properties of graphene-based materials, Chin. Sci. Bull. 57 (2012) 2971-2982.
4. [4]
  [4] M.S. Xu, Y. Gao, X. Yang, H.Z. Chen, Unique synthesis of graphene-based materials for clean energy and biological sensing applications, Chin. Sci. Bull. 57 (2012) 3000-3009.
5. [5]
  [5] H.Y. Hea, J. Klinowskia, M. Forsterb, A. Lerf, A new structural model for graphite oxide, Chem. Phys. Lett. 287 (1998) 53-56.
6. [6]
  [6] P.S. Lau, B.K. Coombes, Y.F. Li, A general approach to the construction of structureswitching reporters from RNA aptamers, Angew. Chem. Int. Ed. 49 (2010) 7938-7942.
7. [7]
  [7] C.H. Lu, H.H. Yang, C.L. Zhu, X. Chen, G.N. Chen, A graphene platform for sensing biomolecules, Angew. Chem. Int. Ed. 48 (2009) 4785-4787.
8. [8]
  [8] S.J. He, B. Song, D. Li, et al., A graphene nanoprobe for rapid, sensitive, and multicolor fluorescent DNA analysis, Adv. Funct. Mater. 20 (2010) 453-459.
9. [9]
  [9] F. Li, Y. Huang, Q. Yang, et al., A graphene-enhanced molecular beacon for homogeneous DNA detection, Nanoscale 2 (2010) 1021-1026.
10. [10]
  [10] Y. Wang, Z.H. Li, D.H. Hu, et al., Aptamer/graphene oxide nanocomplex for in situ molecular probing in living cells, J. Am. Chem. Soc. 132 (2010) 9274-9276.
11. [11]
  [11] H.F. Dong, W.C. Gao, F. Yan, et al., Fluorescence resonance energy transfer between quantum dots and graphene oxide for sensing biomolecules, Anal. Chem. 82 (2010) 5511-5517.
12. [12]
  [12] Y.Q. Wen, F.F. Xing, S.J. He, et al., A graphene-based fluorescent nanoprobe for silver (I) ions detection by using graphene oxide and a silver specific oligonucleotide, Chem. Commun. 46 (2010) 2596-2598.
13. [13]
  [13] S. Tabor, C. Richardson, A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes, Proc. Natl. Acad. Sci. U. S. A. 82 (1985) 1074-1078.
14. [14]
  [14] J.H. Kim, R.G. Larson, Single-molecule analysis of 1D diffusion and transcription elongation of T7 RNA polymerase along individual stretched DNA molecules, Nucleic Acids Res. 35 (2007) 3848-3858.
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