Citation: Pei Jin Xie, Ming Li Ye, Zhong Yang Hu, Guang Wen Pan, Yan Zhu, Jia Jie Zhang. Determination of levels of adenosine phosphates in blood by ion chromatography[J]. Chinese Chemical Letters, ;2011, 22(12): 1485-1488. doi: 10.1016/j.cclet.2011.09.008 shu

Determination of levels of adenosine phosphates in blood by ion chromatography

a Department of Chemistry, Zhejiang University, Hangzhou 310028, China;
b Shanghai Lab, Dionex China Co., Ltd., Shanghai 201203, China

Received Date: 26 May 2011

Fund Project: This research was financially supported by National Natural Science Foundation of China (Nos.20775070 and J0830413), Zhejiang Provincial Natural Science Foundation of China (Nos.R4080124 and Y4090268).

A new method was developed for simultaneous determination of levels of AMP, ADP and ATP in blood by anion-exchange chromatography. The three adenosine phosphates were separated by Dionex IonPac AG18 (50 mm×4 mm) guard column and IonPac AS18 (250 mm×4 mm) analytical column using a gradient method and detected with a suppressed conductivity detector. The detection limits (S/N=3) of AMP, ADP and ATP were 38, 47, 108 ng/L, respectively. The relative standard deviations of retention time, peak area and peak height were all less than 1.87% and a good linear relationship was obtained. This method was applied to analyze human blood samples.
- Adenosine phosphate,
- Ion chromatography,
- Blood
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