Citation: Ai Hua Fu, Zhu Jun Zhang, Li Li Chen, Xiao Ming Zhang, Pan Xue. High performance liquid chromatography with immobilized Ru(bpy)₃²⁺-KMnO_{4 chemiluminescence detection and its application in metabolism of repaglinide in pig liver microsomes[J]. Chinese Chemical Letters,
;2011, 22(10): 1245-1248.

doi:
10.1016/j.cclet.2011.05.023} shu

High performance liquid chromatography with immobilized Ru(bpy)₃²⁺-KMnO_{4 chemiluminescence detection and its application in metabolism of repaglinide in pig liver microsomes}

Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Materials Science, Shaanxi Normal University, Xi'an 710062, China

Received Date: 1 March 2011

Fund Project: This work was supported financially by the Fundamental Research Funds for the Central Universities (No.GK20091004).

A novel high performance liquid chromatography-chemiluminescence (HPLC-CL) method for investigation of in vitro metabolism of repaglinide in pig liver microsomes with microdialysis sampling technique was developed. The chromatographic separation was performed on a Hypersil BDS-C18 column with an isocratic mobile phase consisting of methanol and 0.01 mol/L KH₂PO₄ (pH 3.0) (volume ratio 75:25) at a flow rate of 1.0 mL/min. The detection was based on the chemiluminescence reaction of repaglinide with acidic potassium permanganate (KMnO₄) and tris (2, 2'-bipyridyl)ruthenium (III) (Ru (bpy)₃³⁺), which was immobilized on the cationic ion-exchange resin for obtaining high sensitivity and reducing consumption of expensive reagent.
- High performance liquid chromatography,
- Chemiluminescence,
- Microdialysis,
- Repaglinide,
- Immobilized Ru (bpy)₃²⁺
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