Citation: Yun Yun Zhang, Min Chen, Hong Cui. Isolation and characterization of a new subunit of phycocyanin from Chroomonas placoidea[J]. Chinese Chemical Letters, ;2011, 22(10): 1229-1232. doi: 10.1016/j.cclet.2011.04.007 shu

Isolation and characterization of a new subunit of phycocyanin from Chroomonas placoidea

a College of Chemical and Biological Sciences and Engineering, Yantai University, Yantai 264005, China;
b Yantai Traditional Chinese Medical-science Hospital, Yantai 264002, China

Received Date: 27 December 2010

Fund Project: This work is granted by the National Science Fund of China (No.40976083).

A new phycocyanin (PC) fluorescent subunit named β₂ (18 kDa) was isolated and characterized by both SDS-PAGE and isoelectric focusing (IEF) from a species of cryptophytic alga Chroomonas placoidea. PC was separated and purified by ammonium sulfate sedimentation followed by two steps of Sephadex G-100 chromatography. After denatured in 4 mol/L urea for 48 h, PC was divided into two fractions by passing through a Sephacryl S-100 chromatography column twice. The blue fraction (S-1) contained β subunits with a maximal absorbance at 595 nm in visible light region. While the green fraction (S-2) enriched in α subunits showed a characteristic long wavelength absorbance at 680-700 nm region and exhibited a relatively low molecular weight of 9.4 (α₁) and 8.5 kDa (α₂). Fraction S-1 also consisted of two different fluorescent subunits with molecular weight of 20.1 kDa (β₁) and 18 kDa (β₂) and differed from each other on isoelectric points of pH 5.7 (β₁) and 6.0 (β₂), respectively. Further investigation of peptide sequence will help a lot in elucidating the new subunit β₂ that was smaller in size and more neutral than the known β₁subunit, and may provide an alternative explanation in structure of cryptophytic phycobiliproteins.
- Chroomonas placoidea,
- Phycocyanin subunit,
- Sephacryl S-100 chromatography,
- SDS-PAGE,
- IEF
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