引用本文:
Chang Feng Li, Li Ming Du, Hao Wu, Ying Xia Chang. Determination of L-phenylalanine by cucurbit[7]uril sensitized fluorescence quenching method[J]. Chinese Chemical Letters,
2011, 22(7): 851-854.
doi:
10.1016/j.cclet.2010.12.029
Citation: Chang Feng Li, Li Ming Du, Hao Wu, Ying Xia Chang. Determination of L-phenylalanine by cucurbit[7]uril sensitized fluorescence quenching method[J]. Chinese Chemical Letters, 2011, 22(7): 851-854. doi: 10.1016/j.cclet.2010.12.029
Citation: Chang Feng Li, Li Ming Du, Hao Wu, Ying Xia Chang. Determination of L-phenylalanine by cucurbit[7]uril sensitized fluorescence quenching method[J]. Chinese Chemical Letters, 2011, 22(7): 851-854. doi: 10.1016/j.cclet.2010.12.029
Determination of L-phenylalanine by cucurbit[7]uril sensitized fluorescence quenching method
摘要:
The determination method of L-phenylalanine (LPA) by fluorescence quenching was developed. The assay was based on the combination of the cucurbit[7]uril (CB[7]) with palmatine hydrochloride (PAL) reaction. In the presence of CB[7], the fluorescence of PAL was quenched by LPA which can be employed to detect LPA. Under the optimal conditions, a linear range 3.63×10-8-9.68×10-6 mol/L and a detection limit 1.27×10-8 mol/L of LPA were obtained. The relative standard deviation (R.S.D) was 1.8% obtained from a series of11 standards each containing 6.05×10-6 mol/L oLPA. This paper also discusses the mechanism of fluorescence indicator probe.
English
Determination of L-phenylalanine by cucurbit[7]uril sensitized fluorescence quenching method
Abstract:
The determination method of L-phenylalanine (LPA) by fluorescence quenching was developed. The assay was based on the combination of the cucurbit[7]uril (CB[7]) with palmatine hydrochloride (PAL) reaction. In the presence of CB[7], the fluorescence of PAL was quenched by LPA which can be employed to detect LPA. Under the optimal conditions, a linear range 3.63×10-8-9.68×10-6 mol/L and a detection limit 1.27×10-8 mol/L of LPA were obtained. The relative standard deviation (R.S.D) was 1.8% obtained from a series of11 standards each containing 6.05×10-6 mol/L oLPA. This paper also discusses the mechanism of fluorescence indicator probe.
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Key words:
- L-phenylalanine
- / Palmatine hydrochloride
- / Cucurbit[7]uril
- / Fluorescent probe
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