引用本文:
Xiao Fang Tin, Zhi Li, Shuai Hua Zhang, Chun Xia Wu, Chun Wang, Zhi Wang. Determination of strychnine and brucine in traditional Chinese medicine preparations by capillary zone electrophoresis with micelle to solvent stacking[J]. Chinese Chemical Letters,
2011, 22(3): 330-333.
doi:
10.1016/j.cclet.2010.10.044
Citation: Xiao Fang Tin, Zhi Li, Shuai Hua Zhang, Chun Xia Wu, Chun Wang, Zhi Wang. Determination of strychnine and brucine in traditional Chinese medicine preparations by capillary zone electrophoresis with micelle to solvent stacking[J]. Chinese Chemical Letters, 2011, 22(3): 330-333. doi: 10.1016/j.cclet.2010.10.044
Citation: Xiao Fang Tin, Zhi Li, Shuai Hua Zhang, Chun Xia Wu, Chun Wang, Zhi Wang. Determination of strychnine and brucine in traditional Chinese medicine preparations by capillary zone electrophoresis with micelle to solvent stacking[J]. Chinese Chemical Letters, 2011, 22(3): 330-333. doi: 10.1016/j.cclet.2010.10.044
Determination of strychnine and brucine in traditional Chinese medicine preparations by capillary zone electrophoresis with micelle to solvent stacking
摘要:
A novel micelle to solvent stacking on-line sample preconcentration technique in capillary zone electrophoresis (MSS-CZE) has been developed to determine the strychnine and brucine in traditional Chinese medicine preparations. The optimal running buffer was 30 mmol/L H3PO4 containing 20% acetonitrile at pH 4.0. The sample matrix was 8 mmol/L H3PO4 containing 5 mmol/L sodium dodecyl sulfate (SDS) at pH 3.0. The established MSS-CZE method afforded more than 50-fold improvements in concentration sensitivity compared with typical CZE-UVanalysis. The calibration curve was linear in the range from 0.2 to 15.0 μg/mL for both strychnine and brucine, with correlation coefficients of 0.9984 and 0.9976, respectively. The limits of detection (S/N=3:1) for strychnine and brucine were 0.02 and 0.05 μg/mL, respectively. The MSS-CZE method has been successfully applied to the analysis of strychnine and brucine in Chinese medicinal preparations.
English
Determination of strychnine and brucine in traditional Chinese medicine preparations by capillary zone electrophoresis with micelle to solvent stacking
Abstract:
A novel micelle to solvent stacking on-line sample preconcentration technique in capillary zone electrophoresis (MSS-CZE) has been developed to determine the strychnine and brucine in traditional Chinese medicine preparations. The optimal running buffer was 30 mmol/L H3PO4 containing 20% acetonitrile at pH 4.0. The sample matrix was 8 mmol/L H3PO4 containing 5 mmol/L sodium dodecyl sulfate (SDS) at pH 3.0. The established MSS-CZE method afforded more than 50-fold improvements in concentration sensitivity compared with typical CZE-UVanalysis. The calibration curve was linear in the range from 0.2 to 15.0 μg/mL for both strychnine and brucine, with correlation coefficients of 0.9984 and 0.9976, respectively. The limits of detection (S/N=3:1) for strychnine and brucine were 0.02 and 0.05 μg/mL, respectively. The MSS-CZE method has been successfully applied to the analysis of strychnine and brucine in Chinese medicinal preparations.
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