Citation: Fu Yong Zhao, Zong Hua Wang, Hui Wang, Rui Zhao, Ming Yu Ding. Determination of uric acid in human urine by ion chromatography with conductivity detector[J]. Chinese Chemical Letters, ;2011, 22(3): 342-345. doi: 10.1016/j.cclet.2010.10.008 shu

Determination of uric acid in human urine by ion chromatography with conductivity detector

a Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084, China;
b Laboratory of Fiber Materials and Modern Textile, the Growing Base for State Key Laboratory, Qingdao University, Qingdao 266071, China;
c Metrohm China Ltd. Beijing, Beijing 100005, China

Received Date: 28 June 2010

A simple, fast, precise and eco-friendly analytical method for the determination of uric acid (UA) in human urine by ion chromatography (IC) was established. The sample pretreatment was not required, only needed centrifugation and filtration. The separation was carried out on a cation exchange column with 2.0 mmol/L nitric acid as mobile phase at the flow-rate 1.0 mL/min. A non-suppressed conductivity detector was used. The IC analysis time for one run was within 10 min under the optimized IC condition. The detection limits were 0.5 μg/L(S/N=3) for uric acid. The recovery was 100.1% while the relative standard deviation (RSD) was 1.8% from 10 measurements.
- Ion chromatography,
- Uric acid,
- Urine
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