Citation: Yan Wang, Zhi Hua Chai, Hong Yan He, Yi Zhe Jiang, Guo Qi Fu. Polyacrylamide hydrogels with phenylboronic acid moieties for the imprinting of proteins[J]. Chinese Chemical Letters, ;2010, 21(12): 1487-1489. doi: 10.1016/j.cclet.2010.06.005 shu

Polyacrylamide hydrogels with phenylboronic acid moieties for the imprinting of proteins

Key Laboratory of Functional Polymer Materials of Ministry of Education, Institute of Polymer Chemistry, Nankai University, Tianjin 300071, China

Received Date: 1 April 2010

Fund Project: The work was supported by the National Natural Science Foundation of China (No.20574038) and the Natural Science Foundation of Tianjin (No.09JCYBJC02900).

In this letter, N-acryloyl-3-aminophenylboronic acid (AAPBA) was synthesized and then examined as a new functional monomer for protein imprinting. It was allowed to be copolymerized with acrylamide to produce hemoglobin-or lysozyme-imprinted hydrogels. In template rebinding tests, the imprinted gels showed significant increase in the specific binding with the increase of the AAPBA amounts in the prepolymerization recipes. These results indicate that AAPBA may be a useful functional monomer for its moderate interactions with protein molecules.
- Protein imprinting,
- Hydrogels,
- Hemoglobin,
- Lysozyme,
- 3-Aminophenylboronic acid
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