Citation: Hua Ping Zhu, Ting Ting Liu, Bing Liu, Hui Ling Yin, Xiao Long Li, Li Wang, Shuo Wang. Antigens synthesis and antibodies preparation for furazolidone and its metabolite 3-amino-2-oxazolidinone[J]. Chinese Chemical Letters, ;2010, 21(9): 1049-1052. doi: 10.1016/j.cclet.2010.04.001 shu

Antigens synthesis and antibodies preparation for furazolidone and its metabolite 3-amino-2-oxazolidinone

Key Laboratory of Food Nutrition and Safety(Tianjin University of Science & Technology), Ministry of Education, Tianjin 300457, China

Received Date: 5 January 2010

Fund Project: The authors are grateful for financial supports from the Ministry of Science and Technology of the People's Republic of China (No.2009ZX09502-027 and No.2009BADB9B03).

Two haptens of 3-[(5-amino-furan-2-ylmethylene) amino]oxazolidin-5-one (FZ-NH₂) and 3-{[(4-carboxypbenyl) methylene]-amino}-2-oxazolidinone(CPAOZ)were synthesized. For FZ-NH₂, immunogens were prepared by glutaraldehyde and diazo salt methods. For CPAOZ, immunogens were connected by the methods of the active ester and mixed acid anhydride. Compared with the combination, indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was developed with coating antigen of FZ-NH₂-OVA via the glutaraldehyde method and immunogen of CPAOZ-KLH via active ester method. For furazolidone and its metabolite AOZ (NPAOZ as derivative), the sensitivities (IC₅0) were 2.0 μg/L and 2.5 μg/L, limits of detection (IC₁₅) were0.09 μg/L and 0.25 μg/L, respectively. A sensitive method was developed for the simultaneous determination of furazolidone in feed and its metabolite AOZ in tissue.
- Furzolidone,
- AOZ,
- Antigen,
- Antibody,
- ELISA
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