Citation: Ping Ping Tang, Zhao Feng Luo, Ji Bao Cai, Qing De Su. A novel indirect inhibitive immunoassay for sulfamethoxazole[J]. Chinese Chemical Letters, ;2010, 21(8): 955-958. doi: 10.1016/j.cclet.2010.03.029 shu

A novel indirect inhibitive immunoassay for sulfamethoxazole

a Department of Chemistry, University of Science and Technology of China, Hefei 230026, China;
b Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei 230027, China

Received Date: 22 December 2009

Fund Project: This work is financially supported by National Natural Science Foundation of China (No. 20405013).

A new indirect inhibitive immunoassay using surface plasmon resonance (SPR)coupled with molecularly imprinted polymer (MIP)was developed.A sulfamethoxazole (SMX)MIP coating capillary was produced and used as an in-tube solid phase extraction (SPE)device.The MIP coating formed a nanometer film on the inner wall of the capillary.The anti-SMX mono-antibody was inhibited by SMX extracted by the MIP coating in a dose-dependent manner.The calibration curve was generated by linear fit over the range of 0.04-10.00 ng/mL.The limit of detection was 0.01 ng/mL.This method has high sensitivity and can be performed automatically.
- Sulfamethoxazole,
- Molecularly imprinted polymer,
- Surface plasmon resonance,
- Indirect inhibitive immunoassay
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