Citation: Yan Ming Liu, Zhuan Li Liu. Chemiluminescence determination of 20 amino acids and the mechanism study[J]. Chinese Chemical Letters, ;2010, 21(7): 856-859. doi: 10.1016/j.cclet.2010.01.025 shu

Chemiluminescence determination of 20 amino acids and the mechanism study

College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China

Received Date: 13 November 2009

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 20575056), Henan Innovation Project for University Research Talents (No. 2005126) and Natural Science Foundation of Henan Province of China (No. 092300410122).

It was found that 20 amino acids could inhibit the intensity of the luminol-H₂O₂-CuSO₄ chemiluminescence system.Using this character,a rapid and sensitive method for the determination of 20 amino acids was developed with flow injection coupled with chemiluminescence detection.Under the optimal conditions,the detection limits of 20 amino acids were in the range of 4.5×10^-7-4.3×10^-10 mol/L,and the relative standard deviations were less than 3.2%.The proposed method was successfully applied to drug analysis.The possible mechanism was also discussed.
- Chemiluminescence,
- Flow injection,
- Amino acid,
- Mechanism study
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