Citation: Wen Juan Dong, Jin Ping Song, Chuan Dong, Martin M. F. Choi. Fluorescence quenching method for the determination of catechol with gold nanoparticles and tyrosinase hybrid system[J]. Chinese Chemical Letters, ;2010, 21(3): 346-348. doi: 10.1016/j.cclet.2009.11.043 shu

Fluorescence quenching method for the determination of catechol with gold nanoparticles and tyrosinase hybrid system

a Research Center of Environmental Science and Engineering, College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China;
b Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China

Received Date: 17 August 2009

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 20875059).

The determination method of catechol by fluorescence quenching was developed. The assay was based on the combination of the unique property of gold nanoparticles with tyrosinase enzymatic reaction. In the presence of tyrosinase, the fluorescence of gold nanoparticles was quenched by catechol which can be employed to detect catechol. Under the optimal conditions, a linear range 5.0×10^-7-1.0×10^-3 mol L^-1 and a detection limit 1.0×10^-7 mol L^-1 of catechol were obtained, o-Quinone intermediate produced from the enzymatic catalyzed oxidation of catechol was considered to play the main role in the fluorescence quenching.
- Gold nanoparticles,
- Tyrosinase,
- Catechol,
- Fluorescence quenching
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