Citation: Yan Hua Lai, Yong Nian Ni, Serge Kokot. Authentication of Cassia seeds on the basis of two-wavelength HPLC fingerprinting with the use of chemometrics[J]. Chinese Chemical Letters, ;2010, 21(2): 213-216. doi: 10.1016/j.cclet.2009.10.031 shu

Authentication of Cassia seeds on the basis of two-wavelength HPLC fingerprinting with the use of chemometrics

a State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, 330047, China;
b Department of Chemistry, Nanchang University, Nanchang, 330031, China;
c School of Physical and Chemical Sciences, Queensland University of Technology, Brisbane, Qld 4001, Australia

Received Date: 8 July 2009

Fund Project: The authors gratefully acknowledge the financial support for this study by the National Natural Science Foundation of China (No. NSFC20562009), the Jiangxi Province Natural Science Foundation (No. JXNSF0620041), and the State Key Laboratory of Food Science and Technology of Nanchang University (Nos. SKLF-MB200807 and SKLF-TS200819).

High performance liquid chromatographic(HPLC)fingerprints of Cassia seed,a traditional Chinese medicine(TCM),were developed by means of the chromatograms at two wavelengths of 238 and 282 nm.Then,the two data sets were combined into one matrix.The application of principal component analysis(PCA)for this data matrix showed that the samples were clustered into four groups in accordance with the plant sources and preparation procedures.Furthermore,partial least squares(PLS),back propagation artificial neural network(BP-ANN),and radial basis function artificial neural network(RBF-ANN)were effectively applied to predict the category of the four different samples in the test set.
- Two-wavelength HPLC fingerprinting,
- Cassia seeds,
- Chemometrics,
- Authentication
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