Citation: Xiang Xian Meng, Xiao Hai Yang, Ke Min Wang, Wei Hong Tan, Qiu Ping Guo. Real-time monitoring of DNAzyme cleavage process using fluorescent assay[J]. Chinese Chemical Letters, ;2009, 20(8): 990-994. doi: 10.1016/j.cclet.2009.03.028 shu

Real-time monitoring of DNAzyme cleavage process using fluorescent assay

State Key Laboratory of Chemo/Biosensing and Chemometrics, Engineering Center for Biomedicine, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China

Received Date: 23 December 2008

Fund Project: This work was supported in part by the National Key Basic Research Program of China (No.2002CB513110), Natural Science Foundation of China (No.90606003, No.20505007), Major International (Regional) Joint Research Program of Natural Science Foundation of China (No.20620120107), and project supported by Hunan Provincial Natural Science Foundation of China (No.08JJ1002).

Detection of deoxyribozyme (DNAzyme) cleavage process usually needs complex and time-consuming radial labeling, gel electrophoresis and autoradiography. This paper reported an approach to detect DNAzyme cleavage process in real time using a fluorescence probe. The probe was employed as DNAzyme substrate to convert directly the cleavage information into fluorescence signal in real time. Compared with traditional approach, this non-isotope method not only brought a convenient means to monitor the DNAzyme cleavage reaction, but also offered abundant dynamic data for choosing potential gene therapeutic agents. It provides a new tool for DNAzyme research, as well as a new insight into research on human disease diagnosis. Based on this method, 8-17deoxyribozyme (8-17DNAzyme) against hepatitis C virus RNA (HCV-RNA) was designed and the cleavage process was studied in real time.
- DNAzyme,
- Cleavage,
- Real time,
- HCV-RNA
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