Citation: Han Yu Chen, Hui Sheng Zhuang, Chun Zhou. Development and characterization of new polyclonal antibodies specific for three polychlorinated biphenyls[J]. Chinese Chemical Letters, ;2009, 20(4): 496-500. doi: 10.1016/j.cclet.2008.12.022 shu

Development and characterization of new polyclonal antibodies specific for three polychlorinated biphenyls

a College of Environmental Science and Engineering, DongHua University, Shanghai 201620, China;
b School of Environmental Science and Engineering, Shanghai Jiaotong University, Shanghai 200240, China;
c Department of Environment Engineering, Pingdingshan Institute of Technology, Henan 467000, China

Received Date: 2 June 2008

Fund Project: This work is supported by the National Natural Science Foundation of China (No. 20677008) and The Research Fund for the Doctoral Program of Higher Education (20060255004). And Shanghai Leading Academic Discipline Project (No. B604).

Three polychlorinated biphenyls (PCBs) congeners and their corresponding haptens bearing four carbon length carboxylic groups that can be linked to a protein for raising antibodies were synthesized. The three resultant immunogens were fabricated and used to stimulate immune responses in rabbits to survey the characteristics of the haptens. Three of the resultant polyclonal antibodies (Pabs) were obtained. The antiserum exhibited relatively high antibody titres (1:32-64) in double agar diffusion.
- Immunoassays,
- Polychlorinated biphenyls,
- Haptens,
- Immunogens,
- Polyclonal antibodies
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