Advanced Search

Citation: Zhi Feng Huang, Shan Shan Wang, Chun Yan Ni, Shu Lin Yang, Xiao Kun Li, Susanna S. J. Leong. pH-sensitive polymer-assisted refolding of urea-denatured fibroblast growth factor[J]. Chinese Chemical Letters, ;2009, 20(4): 473-477. doi: 10.1016/j.cclet.2008.12.014 shu

pH-sensitive polymer-assisted refolding of urea-denatured fibroblast growth factor

a School of Chemical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China;
b School of Pharmacy, Wenzhou Medical College, Wenzhou 325035, China;
c School of Chemical and Biomedical Engineering, Nanyang Technological University, 639798 Singapore, Singapore;
d Ministry of Education, Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Jilin 130118, China

Received Date: 2 September 2008

Fund Project: The work was supported by grants from the Hi-tech Research and Development Program of China (No. 2007AA02Z110), Key Grant of Wenzhou Department of Science and Technology (No. Y2007A106), Grant of Guangzhou Department of Science and Technology (No. GK0701013) and Grant from Health Bureau of Zhejiang (No. B135).

A pH-responsive polymer Eudragit S-100 has been found to assist in correct folding of FGF-2(fibroblast growth factor-2) denatured with 8 mol/L urea and 10 mmol/L dithiothreitol at pH 7.2. The refolding of FGF-2 was performed by directly diluting denatured FGF-2 into a refolding buffer containing Eudragit S-100. The ability of Eudragit S-100 to enhance protein refolding level was investigated using MTT method, fluorescence emission spectroscopy and reverse phase HPLC. On the other hand, the result shows the ability of Eudragit S-100 to enhance the refolding level of protein is due to the interaction between Eudragit S-100 and positively charged FGF-2.
- Eudragit S-100,
- FGF-2,
- Refolding
1. [1]
  Chaozheng He , Menghui Xi , Chenxu Zhao , Ran Wang , Ling Fu , Jinrong Huo . Highly N₂ dissociation catalyst: Ir(100) and Ir(110) surfaces. Chinese Chemical Letters, 2025, 36(3): 109671-. doi: 10.1016/j.cclet.2024.109671
2. [2]
  Zhi-Xin Li , Xiao-Feng Qiu , Pei-Qin Liao . Efficient electroreduction of CO₂ to acetate with relative purity of 100% by ultrasmall Cu₂O nanoparticle on a conductive metal-organic framework. Chinese Chemical Letters, 2025, 36(11): 110473-. doi: 10.1016/j.cclet.2024.110473
3. [3]
  Peng Zhang , Yitao Yang , Tian Qin , Xueqiu Wu , Yuechang Wei , Jing Xiong , Xi Liu , Yu Wang , Zhen Zhao , Jinqing Jiao , Liwei Chen . Interface engineering of Pt/CeO₂-{100} catalysts for enhancing catalytic activity in auto-exhaust carbon particles oxidation. Chinese Chemical Letters, 2025, 36(2): 110396-. doi: 10.1016/j.cclet.2024.110396
4. [4]
  Lijun Liao , Yuhao Wang , Yanbo Li , Yingchun Chen , Ruting Yuan , Bo Wang , Tiancheng Zhuang , Guangquan Zhao , Wei Zhou . Synergy of electron-trapping centers and solid adsorption toward 100% suppression of toxic byproduct N₂O in photocatalytic NO reduction. Chinese Chemical Letters, 2026, 37(3): 111398-. doi: 10.1016/j.cclet.2025.111398
5. [5]
  Xiujuan Wang , Yijie Wang , Luyun Cui , Wenqiang Gao , Xiao Li , Hong Liu , Weijia Zhou , Jingang Wang . Coordination-based synthesis of Fe single-atom anchored nitrogen-doped carbon nanofibrous membrane for CO₂ electroreduction with nearly 100% CO selectivity. Chinese Chemical Letters, 2024, 35(12): 110031-. doi: 10.1016/j.cclet.2024.110031
6. [6]
  Weihong Ding , Kaiyue Song , Xianglong Li , Xiaoxia Sun . High-temperature-stable RRAMs with well-defined thermal effect mechanisms enable by engineering of robust 2D <100>-oriented organic-inorganic hybrid perovskites. Chinese Chemical Letters, 2025, 36(4): 110495-. doi: 10.1016/j.cclet.2024.110495
7. [7]
  Hongrui Zhang , Miaoying Cui , Yongjie Lv , Yongfang Rao , Yu Huang . A short review on research progress of ZnIn₂S₄-based S-scheme heterojunction: Improvement strategies. Chinese Chemical Letters, 2025, 36(4): 110108-. doi: 10.1016/j.cclet.2024.110108
8. [8]
  Ziyi Xiao , Xinyi Ma , Linping Wang , Haobin Hu , Enzhou Liu . Efficient photocatalytic conversion H₂S over NiS₂/twinned-Mn_0.5Cd_0.5S Schottky/S-scheme homojunction in Na₂S/Na₂SO₃ solution. Acta Physico-Chimica Sinica, 2026, 42(4): 100171-0. doi: 10.1016/j.actphy.2025.100171
9. [9]
  Xibao Li , Yiyang Wan , Fang Deng , Yingtang Zhou , Pinghua Chen , Fan Dong , Jizhou Jiang . Advances in Z-scheme and S-scheme heterojunctions for photocatalytic and photoelectrocatalytic H₂O₂ production. Chinese Chemical Letters, 2025, 36(10): 111418-. doi: 10.1016/j.cclet.2025.111418
10. [10]
  Haoyuan Qin , Lijing Wang , Yuanhao Tang , Weilong Shi , Changyu Lu . Modulating charge kinetics in CDs/CTF S-scheme hybrids for enhanced H₂O₂ photosynthesis. Chinese Journal of Structural Chemistry, 2026, 45(4): 100858-100858. doi: 10.1016/j.cjsc.2025.100858
11. [11]
  Maomao Liu , Guizeng Liang , Ningce Zhang , Tao Li , Lipeng Diao , Ping Lu , Xiaoliang Zhao , Daohao Li , Dongjiang Yang . Electron-rich Ni2+ in Ni3S2 boosting electrocatalytic CO2 reduction to formate and syngas. Chinese Journal of Structural Chemistry, 2024, 43(8): 100359-100359. doi: 10.1016/j.cjsc.2024.100359
12. [12]
  Xiaohan Wang , Xue Guo , Zhuo Song , Chen Guan , Chengyu Yang , Tianyang Li , Yukun Zhu , Yan Xu . Interface engineering of Co9S8/Ni3S2 heterojunction with robust stability for boosting peroxymonosulfate activation. Chinese Journal of Structural Chemistry, 2026, 45(2): 100771-100771. doi: 10.1016/j.cjsc.2025.100771
13. [13]
  Qiuwen Wang , Linlin Liang , Zexun Li , Jinyu Zhu , Kang Wang , Fuqiang Zhang , Zhijun Ren , Pengfei Wang . Constructing Fe-S bond enhanced charge transfer in α-Fe₂O₃/Cd_0.9Zn_0.1S S-scheme heterojunction for simultaneous oxidation of ciprofloxacin and reduction of Cr(Ⅵ). Chinese Chemical Letters, 2026, 37(6): 112066-. doi: 10.1016/j.cclet.2025.112066
14. [14]
  Yilin Cui , Zhongfu Li , Weikun Zhang , Yu Gao , Zhichao Dong , Conghua Liu . Sulfur vacancies boosting C₂H₄ selectivity of 2D/2D In_2.77S₄/CuInS₂ S-scheme heterojunction for CO₂ photoreduction. Acta Physico-Chimica Sinica, 2026, 42(7): 100274-. doi: 10.1016/j.actphy.2026.100274
15. [15]
  Linfeng Xiao , Wanlu Ren , Shishi Shen , Mengshan Chen , Runhua Liao , Yingtang Zhou , Xibao Li . Enhancing Photocatalytic Hydrogen Evolution through Electronic Structure and Wettability Adjustment of ZnIn₂S₄/Bi₂O₃ S-Scheme Heterojunction. Acta Physico-Chimica Sinica, 2024, 40(8): 2308036-0. doi: 10.3866/PKU.WHXB202308036
16. [16]
  Zheng Liu , Yuqing Bian , Graham Dawson , Jiawei Zhu , Kai Dai . Rational constructing of Zn_0.5Cd_0.5S-diethylenetriamine/g-C₃N₄ S-scheme heterojunction with enhanced photocatalytic H₂O₂ production. Chinese Chemical Letters, 2025, 36(9): 111272-. doi: 10.1016/j.cclet.2025.111272
17. [17]
  Chengxin Chen , Hongfei Shi , Xiaoyan Cai , Liang Mao , Zhe Chen . Enhanced bifunctional photocatalytic performances for H₂ evolution and HCHO elimination with an S-scheme CoWO₄/CdIn₂S₄ heterojunction. Acta Physico-Chimica Sinica, 2025, 41(12): 100155-0. doi: 10.1016/j.actphy.2025.100155
18. [18]
  Jiali Lei , Juan Wang , Wenhui Zhang , Guohong Wang , Zihui Liang , Jinmao Li . TiO₂/CdIn₂S₄ S-scheme heterojunction photocatalyst promotes photocatalytic hydrogen evolution coupled vanillyl alcohol oxidation. Acta Physico-Chimica Sinica, 2025, 41(12): 100174-0. doi: 10.1016/j.actphy.2025.100174
19. [19]
  Ze Luo , Yukun Zhu , Yadan Luo , Guangmin Ren , Yonghong Wang , Hua Tang . Photocatalytic selective oxidation of 5-hydroxymethylfurfural coupled with H₂ evolution over In₂O₃/ZnIn₂S₄ S-scheme heterojunction. Acta Physico-Chimica Sinica, 2026, 42(3): 100166-0. doi: 10.1016/j.actphy.2025.100166
20. [20]
  Mengru , Qu , Chuanzhi Sun , Shujuan Jiang , Shaoqing Song . Localized Co3O4/In2S3 S-scheme heterojunction by polarization engineering for achieving an exceptional H2 efficiency. Chinese Journal of Structural Chemistry, 2026, 45(5): 100870-100870. doi: 10.1016/j.cjsc.2026.100870

Metrics

PDF Downloads(0)
Abstract views(1673)
HTML views(82)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Address：Zhongguancun North First Street 2,100190 Beijing, PR China Tel: +86-010-82449177-888

Powered By info@rhhz.net

/

DownLoad: Full-Size Img PowerPoint