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Citation: Zhi Feng Huang, Shan Shan Wang, Chun Yan Ni, Shu Lin Yang, Xiao Kun Li, Susanna S. J. Leong. pH-sensitive polymer-assisted refolding of urea-denatured fibroblast growth factor[J]. Chinese Chemical Letters, ;2009, 20(4): 473-477. doi: 10.1016/j.cclet.2008.12.014 shu

pH-sensitive polymer-assisted refolding of urea-denatured fibroblast growth factor

a School of Chemical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China;
b School of Pharmacy, Wenzhou Medical College, Wenzhou 325035, China;
c School of Chemical and Biomedical Engineering, Nanyang Technological University, 639798 Singapore, Singapore;
d Ministry of Education, Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Jilin 130118, China

Received Date: 2 September 2008

Fund Project: The work was supported by grants from the Hi-tech Research and Development Program of China (No. 2007AA02Z110), Key Grant of Wenzhou Department of Science and Technology (No. Y2007A106), Grant of Guangzhou Department of Science and Technology (No. GK0701013) and Grant from Health Bureau of Zhejiang (No. B135).

A pH-responsive polymer Eudragit S-100 has been found to assist in correct folding of FGF-2(fibroblast growth factor-2) denatured with 8 mol/L urea and 10 mmol/L dithiothreitol at pH 7.2. The refolding of FGF-2 was performed by directly diluting denatured FGF-2 into a refolding buffer containing Eudragit S-100. The ability of Eudragit S-100 to enhance protein refolding level was investigated using MTT method, fluorescence emission spectroscopy and reverse phase HPLC. On the other hand, the result shows the ability of Eudragit S-100 to enhance the refolding level of protein is due to the interaction between Eudragit S-100 and positively charged FGF-2.
- Eudragit S-100,
- FGF-2,
- Refolding
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