引用本文:
Yu Dong Shen, Yu Wang, Shi Wei Zhang, Zhi Li Xiao, Yuan Ming Sun, Xian Zhang Bu, Lian Quan Gu. Design and efficient synthesis of novel haptens and complete antigens for the AOZ,a toxic metabolite of furazolidone[J]. Chinese Chemical Letters,
2007, 18(12): 1490-1492.
doi:
10.1016/j.cclet.2007.10.030
Citation: Yu Dong Shen, Yu Wang, Shi Wei Zhang, Zhi Li Xiao, Yuan Ming Sun, Xian Zhang Bu, Lian Quan Gu. Design and efficient synthesis of novel haptens and complete antigens for the AOZ,a toxic metabolite of furazolidone[J]. Chinese Chemical Letters, 2007, 18(12): 1490-1492. doi: 10.1016/j.cclet.2007.10.030
Citation: Yu Dong Shen, Yu Wang, Shi Wei Zhang, Zhi Li Xiao, Yuan Ming Sun, Xian Zhang Bu, Lian Quan Gu. Design and efficient synthesis of novel haptens and complete antigens for the AOZ,a toxic metabolite of furazolidone[J]. Chinese Chemical Letters, 2007, 18(12): 1490-1492. doi: 10.1016/j.cclet.2007.10.030
Design and efficient synthesis of novel haptens and complete antigens for the AOZ,a toxic metabolite of furazolidone
摘要:
A good strategy was brought forward for designing efficient haptens and complete antigens for 3-amino-2-oxazolidinone (AOZ). Haptens designed newly were achieved facilely in good yield by using LiCl-N (Et)3 as new catalysis system, the structure of which was elucidated by spectroscopy analysis, such as NMR and MS. Novel antigens for AOZ were prepared successfully by convenient ester method. The ratios of haptens 3 and 4 to carrier proteins were proven respectively as 41:1 (5a), 39:1 (6a), 11:1 (5b) and 9:1 (6b) by trinitrobenzene sulfonic acid (TNBS) method. The results of indirect competitive ELISA (ic-ELISA) of antiserums indicated that the haptens with a short unsaturated side chain can evoke specific immune response effectively.
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关键词:
- Furazolidone
- / AOZ
- / Antigen
- / Hapten
- / Immunoassay
English
Design and efficient synthesis of novel haptens and complete antigens for the AOZ,a toxic metabolite of furazolidone
Abstract:
A good strategy was brought forward for designing efficient haptens and complete antigens for 3-amino-2-oxazolidinone (AOZ). Haptens designed newly were achieved facilely in good yield by using LiCl-N (Et)3 as new catalysis system, the structure of which was elucidated by spectroscopy analysis, such as NMR and MS. Novel antigens for AOZ were prepared successfully by convenient ester method. The ratios of haptens 3 and 4 to carrier proteins were proven respectively as 41:1 (5a), 39:1 (6a), 11:1 (5b) and 9:1 (6b) by trinitrobenzene sulfonic acid (TNBS) method. The results of indirect competitive ELISA (ic-ELISA) of antiserums indicated that the haptens with a short unsaturated side chain can evoke specific immune response effectively.
-
Key words:
- Furazolidone
- / AOZ
- / Antigen
- / Hapten
- / Immunoassay
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