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Citation:
Wu Yong Sun, Wen Ying Liu, Ling Bo Qu. Development of ELISA and immunochromatographic assay for ofloxacin[J]. Chinese Chemical Letters,
;2007, 18(9): 1107-1110.
doi:
10.1016/j.cclet.2007.07.008
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Two rapid, sensitive and reliable immunoassay methods, namely competitive indirect enzyme-linked immunosorbent assay (CIELISA) and colloidal gold-based immunochromatographic assay (CGIA), were developed to detect ofloxacin (OFL). The linear range of the CI-ELISA was from 0.5 to 128 ng/mL with a limit of detection (LOD) of 0.35 ng/mL. Good recoveries were obtained in analyzing simulated swine urine samples. The CGIA could accurately estimate OFL at concentrations as low as 10 ng/mL in less than 10 min, and test results were read visually without any instrument.
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