Citation: Lian Hui Chen, Yi Jian, Hong Qun Luo, Shao Pu Liu, Xiao Li Hu. Determination of sodium hyaluronate with some basic bisphenylnaphthylmethane dyes by resonance Rayleigh scattering method[J]. Chinese Chemical Letters, ;2007, 18(9): 1099-1102. doi: 10.1016/j.cclet.2007.06.020 shu

Determination of sodium hyaluronate with some basic bisphenylnaphthylmethane dyes by resonance Rayleigh scattering method

a Department of Chemistry, North Sichuan Medical College, Nanchong 637007, China;
b Power Supply Shop, Jiajiang Hydraulic Machinery Works, Leshan 614000, China;
c College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China

Received Date: 4 April 2007

Fund Project: We are grateful for financial support from the Sichuan Province Education Foundation (No.2003B013),Chongqing Scientific and Technical Committee Foundation (No.2002-7472) and from North Sichuan Medical College (No.2006-15).

In an acetic acid-sodium acetate buffer solution of pH 3.6-6.8, a compound complex was formed between sodium hyaluronate (abbreviated as SH) and some basic bisphenylnaphthylmethane dyes, leading to a great enhancement of the intensity of resonance Rayleigh scattering (RRS) and giving a new RRS spectrum, with its maximum scattering peak near 280 nm. It was also found that the intensity of RRS was directly proportional to the concentration of SH near the range between 0 and 3.0 mg/L. Based on these facts, a sensitive method for the determination of SH has been established. The method had good selectivity, and has been used for the determination of total amounts of SH in samples with satisfactory results. For the NB-SH system, the detection limit of SH was down to 13.7 ng/mL.
- Resonance reyleigh scattering (RRS),
- Sodium hyaluronate,
- Basic bisphenylnaphthylmethane dyes,
- Determination
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