引用本文:
Xiao Yan Zhang, Quan Ling Suo, Xiong Hui Wei. A spectral study of the interaction between chelerythrine chloride and cytidine[J]. Chinese Chemical Letters,
2007, 18(6): 722-725.
doi:
10.1016/j.cclet.2007.04.023
Citation: Xiao Yan Zhang, Quan Ling Suo, Xiong Hui Wei. A spectral study of the interaction between chelerythrine chloride and cytidine[J]. Chinese Chemical Letters, 2007, 18(6): 722-725. doi: 10.1016/j.cclet.2007.04.023
Citation: Xiao Yan Zhang, Quan Ling Suo, Xiong Hui Wei. A spectral study of the interaction between chelerythrine chloride and cytidine[J]. Chinese Chemical Letters, 2007, 18(6): 722-725. doi: 10.1016/j.cclet.2007.04.023
A spectral study of the interaction between chelerythrine chloride and cytidine
摘要:
To study the possible anticancer mechanisms of chelerythrine (CHE), and its interactions with cytidine were investigated by UV-vis spectrophotometric and spectrofluorimetric measurements and by thermodynamic calculations.The binding of CHE to cytidine could be characterized by the hypochromic and bathochromic effects in the absorption bands, and the quenching of fluorescence intensity.The spectral data were fit by linear analysis, yielding a binding constant of 2.49×104 L mol-1at 25℃ of CHE and cytidine, and a van't Hoff enthalpy of -20.02 kJ/mol for the exothermic interaction in the standard state.In addition, with △rGMΘ=-25.09 kJ/mol and △rSmΘ=17.01 J/molK, the interactions should be entropy-driven.
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关键词:
- Chelerythrine
- / Cytidine
- / Fluorescence quenching
- / UV
- / Thermodynamic
English
A spectral study of the interaction between chelerythrine chloride and cytidine
Abstract:
To study the possible anticancer mechanisms of chelerythrine (CHE), and its interactions with cytidine were investigated by UV-vis spectrophotometric and spectrofluorimetric measurements and by thermodynamic calculations.The binding of CHE to cytidine could be characterized by the hypochromic and bathochromic effects in the absorption bands, and the quenching of fluorescence intensity.The spectral data were fit by linear analysis, yielding a binding constant of 2.49×104 L mol-1at 25℃ of CHE and cytidine, and a van't Hoff enthalpy of -20.02 kJ/mol for the exothermic interaction in the standard state.In addition, with △rGMΘ=-25.09 kJ/mol and △rSmΘ=17.01 J/molK, the interactions should be entropy-driven.
-
Key words:
- Chelerythrine
- / Cytidine
- / Fluorescence quenching
- / UV
- / Thermodynamic
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