Citation: Yong Nian Ni, Xue Zhi Zhong. Investigation of the interaction of DNA and neutral red by fluorescence spectroscopic analysis[J]. Chinese Chemical Letters, ;2007, 18(5): 569-572. doi: 10.1016/j.cclet.2007.03.027 shu

Investigation of the interaction of DNA and neutral red by fluorescence spectroscopic analysis

Yong Nian Ni ^a,b, ,
Xue Zhi Zhong ^a

a Department of Chemistry, Nanchang University, Nanchang 330047, China;
b The Key Laboratory of Food Science of MOE, Nanchang University, Nanchang 330047, China

Received Date: 4 January 2007

Fund Project: The financial support from the National Natural Science Foundation of China (No. 20562009), the Natural Science Foundation of Jiangxi Province (No. 0620041), and the foundation of the State Key Laboratories of the Chemo/Biosensing and Chemometrics of Hunan University (No. 2005-22), and the program for Changjiang Scholars and Innovative Research Team in Universities (No. IRT0540).

The binding characteristics of neutral red (NR) with DNA were investigated by fluorescence spectrometry. Chemometrics approach as singular value decomposition (SVD) was used to evaluate the number of spectral species in the drug-DNA binding process, and then the intrinsic binding constant of 1.6×10⁴ in base pairs and the binding site number of 0.97 were obtained from the Scatchard plot.
- Neutral red,
- DNA,
- Binding constant,
- Fluorescence,
- Chemometrics,
- Singular value decomposition
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