Citation: Jiang Jiang, Yu Hua Mei, Long Gen Zhu, Wen Ji Wang. Site-specifically cleavage of oxidized insulin B chain with Zn(Ⅱ) ion studied by electrospray ionization mass spectrometry[J]. Chinese Chemical Letters, ;2007, 18(5): 557-560. doi: 10.1016/j.cclet.2007.03.016 shu

Site-specifically cleavage of oxidized insulin B chain with Zn(Ⅱ) ion studied by electrospray ionization mass spectrometry

a College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350002, China;
b College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
c State Key Laboratory of Coordination Chemistry, Nanjing University, Nanjing 210093, China

Received Date: 13 November 2006

The electrospray ionization mass spectrometry and tandem mass spectrometry investigation showed that the binding sites of Zn²⁺ with oxidized insulin B chain are His 5, His 10, and Arg 22, which lead to the selective cleavages of the peptide bonds at Asn 3-Gln 4, His 5-Leu 6, Gly 8-Ser 9, and Glu 21-Arg 22 of oxidized insulin B chain.
- Oxidized insulin B chain,
- ZnCl₂,
- Mass spectrometry,
- Hydrolytic cleavage
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