Citation: Wan Gen Yang, Zhang Wang, Shi Ying Xu. A new method for determination of antithrombotic activity of egg white protein hydrolysate by microplate reader[J]. Chinese Chemical Letters, ;2007, 18(4): 449-451. doi: 10.1016/j.cclet.2007.02.014 shu

A new method for determination of antithrombotic activity of egg white protein hydrolysate by microplate reader

School of Food Science & Technology, Southern Yangtze University, Wuxi 214036, China

Received Date: 30 October 2006

A new method for the determination of antithrombotic activity of egg white protein hydrolysate (EWPH) was developed using a microplate reader. Reaction was carried out at 37℃ and pH 7.2 with fibrinogen concentration 0.1%. Microplate reading was conducted at 405 nm. Inhibition rate of EWPH on thrombin activity showed linearity (R²=0.9971), when the inhibition rate was in the range of 10-90%. The lower limit of detection (LLD, at 99.7% probability) and the biological limit of detection (BLD, at 99.7% probability) of the method were 10.643 and 40 mg/mL, respectively. The repeatability standard deviation (R.S.D.) was 1.08%. The standard deviation of the method was±0.027 AT-U.
- Egg white protein hydrolysate,
- Antithrombotic activity,
- Microplate reader
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