Citation: Cheng Quan Wang, Hui Wang, Yan Ming Liu. Capillary electrophoresis with direct chemiluminescence detection for the analysis of catecholamines in human urine[J]. Chinese Chemical Letters, ;2007, 18(4): 452-454. doi: 10.1016/j.cclet.2007.02.009 shu

Capillary electrophoresis with direct chemiluminescence detection for the analysis of catecholamines in human urine

College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China

Received Date: 29 November 2006

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 20575056),the Outstanding Youth Science Foundation of Henan Province (No. 04120001300),and Henan Innovation Project for University Research Talents (No. 2005126).

A rapid and sensitive method for the analysis of three catecholamines by capillary electrophoresis (CE) with direct chemiluminescence (CL) detection is described. The detection limits (S/N=3) were 1.3×10^-8 g/mL for isoprenaline,1.0×10^-8 g/mL for epinephrine and 2.8×10^-8 g/mL for dopamine. The proposed method was successfully applied to the analysis of catecholamines in urine samples of cigarette smokers and nonsmokers. The results showed that there is a close relation between the release of dopamine in human body fluids and cigarette smoking/nonsmoking.
- Capillary electrophoresis,
- Catecholamines,
- Chemiluminescence detection,
- Urine
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