引用本文:
Jian Quan Lu, Ming Bo Xu, Xing Wang Zhou, Jin Guang Xu, Qing Tao. A novel detection of single-stranded DNA binding protein based on ss-DNA modified chip using surface plasmon resonance microscopy[J]. Chinese Chemical Letters,
2007, 18(4): 441-444.
doi:
10.1016/j.cclet.2007.01.012
Citation: Jian Quan Lu, Ming Bo Xu, Xing Wang Zhou, Jin Guang Xu, Qing Tao. A novel detection of single-stranded DNA binding protein based on ss-DNA modified chip using surface plasmon resonance microscopy[J]. Chinese Chemical Letters, 2007, 18(4): 441-444. doi: 10.1016/j.cclet.2007.01.012
Citation: Jian Quan Lu, Ming Bo Xu, Xing Wang Zhou, Jin Guang Xu, Qing Tao. A novel detection of single-stranded DNA binding protein based on ss-DNA modified chip using surface plasmon resonance microscopy[J]. Chinese Chemical Letters, 2007, 18(4): 441-444. doi: 10.1016/j.cclet.2007.01.012
A novel detection of single-stranded DNA binding protein based on ss-DNA modified chip using surface plasmon resonance microscopy
摘要:
An ss-DNA gold chip was prepared based on self-assembly of the thiol-derivatized oligonucleotide, and used for the determination of single-stranded binding protein (SSB) by surface plasmon resonance microscopy (SPR). The experiment results showed that SSB binds ss-DNA with high specificity, and relative signal of SPR response is proportional to the concentration of SSB in the range of 0.1-100 ng/mL with a detection limit (S/N=3) of 0.07 ng/mL.
English
A novel detection of single-stranded DNA binding protein based on ss-DNA modified chip using surface plasmon resonance microscopy
Abstract:
An ss-DNA gold chip was prepared based on self-assembly of the thiol-derivatized oligonucleotide, and used for the determination of single-stranded binding protein (SSB) by surface plasmon resonance microscopy (SPR). The experiment results showed that SSB binds ss-DNA with high specificity, and relative signal of SPR response is proportional to the concentration of SSB in the range of 0.1-100 ng/mL with a detection limit (S/N=3) of 0.07 ng/mL.
扫一扫看文章
计量
- PDF下载量: 0
- 文章访问数: 1696
- HTML全文浏览量: 129
下载:
