Citation: Xing Chen, Lu Yang, Wangcheng Zhan, Li Wang, Yun Guo, Yunsong Wang, Guanzhong Lu, Yanglong Guo. Immobilization of penicillin G acylase on paramagnetic polymer microspheres with epoxy groups[J]. Chinese Journal of Catalysis, ;2018, 39(1): 47-53. doi: 10.1016/S1872-2067(17)62934-6 shu

Immobilization of penicillin G acylase on paramagnetic polymer microspheres with epoxy groups

Key Laboratory for Advanced Materials and Research Institute of Industrial Catalysis, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China

Received Date: 22 August 2017
Revised Date: 13 October 2017

Fund Project: This work was supported by the National Natural Science Foundation of China (91545103), Shu Guang Project of Shanghai Municipal Education Commission and Shanghai Education Development Foundation (10SG30), and the Fundamental Research Funds for the Central Universities (222201717003).

Paramagnetic polymer microspheres were synthesized by the inverse suspension polymerization method through polymerization of glycidyl methacrylate, ally glycidyl ether and methacrylamide on the surface of silica-coated Fe₃O₄ nanoparticles using N,N'-methylene-bis(acrylamide) as a cross-linking agent. Penicillin G acylase (PGA) was covalently immobilized on the surface of the paramagnetic microspheres by reacting the amino groups of the PGA molecules with the epoxy groups of the paramagnetic polymer microspheres. The effect of the SiO₂ coating and the amount of paramagnetic Fe₃O₄ nanoparticles on the initial activity and the operational stability of the immobilized PGA was investigated. The results indicated that SiO₂ played an important role in the polymerization process and paramagnetic polymer microspheres with a SiO₂-coated Fe₃O₄ nanoparticles mass content of 7.5% are an optimal support material for PGA immobilization. Immobilized PGA on the paramagnetic polymer microspheres shows a high initial activity of 430 U/g (wet) and retains 99% of its initial activity after recycling 10 times. Furthermore, immobilized PGA exhibits high thermal stability, pH stability and excellent reusability, which can be rapidly recycled by the aid of magnet.
- Paramagnetic polymer microspheres,
- Epoxy groups,
- Penicillin G acylase,
- Covalent bonding,
- Immobilization
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