Citation: Qikun Zhang, Junqing Kang, Bing Yang, Leizhen Zhao, Zhaosheng Hou, Bo Tang. Immobilized cellulase on Fe₃O₄ nanoparticles as a magnetically recoverable biocatalyst for the decomposition of corncob[J]. Chinese Journal of Catalysis, ;2016, 37(3): 389-397. doi: 10.1016/S1872-2067(15)61028-2 shu

Immobilized cellulase on Fe₃O₄ nanoparticles as a magnetically recoverable biocatalyst for the decomposition of corncob

1.
College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Normal University, Jinan 250014, Shandong, China

Corresponding author: Qikun Zhang,
Received Date: 2 December 2015
Available Online: 8 December 2015
Fund Project: 国家重点基础研究发展计划(973计划,2013CB933800) (973计划,2013CB933800)山东省自然科学基金(ZR2013EMM004) (ZR2013EMM004)济南市高校自主创新计划(201401245) (201401245)国家级大学生创新训练项目(201410445074). (201410445074)

A magnetically recoverable biocatalyst was successfully prepared through the immobilization of cellulase onto Fe₃O₄ nanoparticles. The magnetic nanoparticles were synthesized by a hydrothermal method in an aqueous system. The support (Fe₃O₄ nanoparticles) was modified with (3-aminopropyl)triethoxysilane, and glutaraldehyde was used as the cross-linker to immobilize the cellulose onto the modified support. Different factors that influence the activity of the immobilized enzyme were investigated. The experimental results indicated that the suitable immobilization temperature and pH are 40 ℃ and 6.0, respectively. The optimal glutaraldehyde concentration is ~2.0 wt%, and the appropriate immobilization time is 4 h. Under these optimal conditions, the activity of the immobilized enzyme could be maintained at 99.1% of that of the free enzyme. Moreover, after 15 cyclic runs, the activity of the immobilized enzyme was maintained at ~91.1%. The prepared biocatalyst was used to decompose corncobs, and the maximum decomposition rate achieved was 61.94%.
- Magnetic nanoparticle,
- Cellulase,
- Enzyme immobilization,
- Corncob,
- Glutaraldehyde
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沈阳化工大学材料科学与工程学院沈阳 110142

Immobilized cellulase on Fe3O4 nanoparticles as a magnetically recoverable biocatalyst for the decomposition of corncob

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Immobilized cellulase on Fe₃O₄ nanoparticles as a magnetically recoverable biocatalyst for the decomposition of corncob