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Citation: GUO Su, XUE Mian-Qi, QIAN Min-Xie, CAO Ting-Bing, ZHAO Xin-Sheng. Selective Protein Immobilization via DNA Conjugation under Microfluidic Laminar Flow[J]. Acta Physico-Chimica Sinica, ;2007, 23(12): 1827-1830. doi: 10.1016/S1872-1508(07)60085-0 shu

Selective Protein Immobilization via DNA Conjugation under Microfluidic Laminar Flow

1.
Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, P. R. China; Department of Chemistry, Renmin University of China, Beijing 100872, P. R. China

Received Date: 22 August 2007
Available Online: 16 October 2007

The feasibility of controlled protein immobilization via DNA conjugation by utilizing laminar flow in a microfluidic device was demonstrated. The glass surface in a microchannel was treated by oli nucleotides. The laminar flow brought different protein-DNA conjugates parallel into the microchannel. DNA recognition allows proteins to be delivered to the desired location. The total internal reflection fluorescence was also applied to monitor the process. Both the specificity and sensitivity were high, and the immobilization and removal of the proteins were repeatable. It was shown that with parallel detection of specific and non-specific recognitions, the accuracy of bio-assay would be effectively enhanced. This strategy could improve the performing quality of biosensors in microfluidic devices.
- Microfluidic device; Protein sensor; DNA-directed immobilization; Total internal reflection fluorescence
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