注册 English

Protein separation using a novel silica-based RPLC/IEC stationary phase modified with N-methylimidazolium ionic liquid

Yi-Xin Wang ,  Kai-Lou Zhao ,  Fan Yang ,  Lei Tian ,  Ying Yang ,  Quan Bai

downloadPDF
Citation:  Yi-Xin Wang, Kai-Lou Zhao, Fan Yang, Lei Tian, Ying Yang, Quan Bai. Protein separation using a novel silica-based RPLC/IEC stationary phase modified with N-methylimidazolium ionic liquid[J]. Chinese Chemical Letters, 2015, 26(8): 988-992. doi: 10.1016/j.cclet.2015.05.001 shu

Protein separation using a novel silica-based RPLC/IEC stationary phase modified with N-methylimidazolium ionic liquid

    • 通讯作者: Quan Bai,
  • 基金项目:

    This work is supported by the National 863 Program (No. 2006AA02Z227) (No. 2006AA02Z227)

    Natural Science Foundation of Shaanxi Province (No. 2011JZ002) (No. 2011JZ002)

    the Foundation of Key Laboratory in Shaanxi Province (Nos. 2010JS103, 11JS097, 14JS098) (Nos. 2010JS103, 11JS097, 14JS098)

    Technology Co-ordinating innovation projects (No. 2013SZS18-K01). (No. 2013SZS18-K01)

摘要: Ionic liquids (ILs) immobilized on silica as novel high performance liquid chromatography (HPLC) stationary phases have attracted considerable attention. However, it has not been applied to protein separation. In this paper, N-methylimidazolium IL-modified silica-based stationary phase (SilprMim) was prepared and investigated as a novel multi-interaction stationary phase charged positively for protein separation. The results indicate that all of the basic proteins tested cannot be absorbed on this novel stationary phase, whereas all of the acidic proteins tested can be retained, and the baseline separation of eight kinds of acidic protein standards can be achieved when performed in reversed phase/ion-exchange chromatography (RPLC/IEC) mode. Compared with commonly used commercial octadecylated silica (ODS) column, the novel stationary phase can show selectivity and good resolution to acidic proteins, which has a promising application in the separation and analyses of acidic proteins from the complex samples in proteomics. In addition, the chromatographic behavior of proteins, the effect of the ligand structure and the retention mechanism on this stationary phase were also investigated.

English

  • 

    1. [1] O. Núñez, K. Nakanishi, N. Tanaka, Preparation of monolithic silica columns for high-performance liquid chromatography, J. Chromatogr. A 1191 (2008) 231-252.[1] O. Núñez, K. Nakanishi, N. Tanaka, Preparation of monolithic silica columns for high-performance liquid chromatography, J. Chromatogr. A 1191 (2008) 231-252.

    2. [2] H.D. Qiu, X.J. Liang, M. Sun, S.X. Jiang, Development of silica-based stationary phases for high-performance liquid chromatography, Anal. Bioanal. Chem. 399 (2011) 3307-3322.[2] H.D. Qiu, X.J. Liang, M. Sun, S.X. Jiang, Development of silica-based stationary phases for high-performance liquid chromatography, Anal. Bioanal. Chem. 399 (2011) 3307-3322.

    3. [3] J.J. Kirkland, J.J. DeStefano, The art and science of forming packed analytical highperformance liquid chromatography columns, J. Chromatogr. A 1126 (2006) 50-57.[3] J.J. Kirkland, J.J. DeStefano, The art and science of forming packed analytical highperformance liquid chromatography columns, J. Chromatogr. A 1126 (2006) 50-57.

    4. [4] J.J. Kirkland, Development of some stationary phases for reversed-phase HPLC, J. Chromatogr. A 1060 (2004) 9-21.[4] J.J. Kirkland, Development of some stationary phases for reversed-phase HPLC, J. Chromatogr. A 1060 (2004) 9-21.

    5. [5] Y.Y. Li, S.Y. Cheng, P.C. Dai, X.M. Liang, Y.X. Ke, Large-pore monodispersed mesoporous silica spheres: synthesis and application in HPLC, Chem. Commun. 9 (2009) 1085-1087.[5] Y.Y. Li, S.Y. Cheng, P.C. Dai, X.M. Liang, Y.X. Ke, Large-pore monodispersed mesoporous silica spheres: synthesis and application in HPLC, Chem. Commun. 9 (2009) 1085-1087.

    6. [6] T.L. Greaves, C.J. Drummond, Protic ionic liquids: properties and applications, Chem. Rev. 108 (2008) 206-237.[6] T.L. Greaves, C.J. Drummond, Protic ionic liquids: properties and applications, Chem. Rev. 108 (2008) 206-237.

    7. [7] X.H. Xiao, S.J. Liu, X. Liu, S.X. Jiang, Application of ionic liquid in analytical chemistry, Chin. J. Anal. Chem. 4 (2005) 569-574.[7] X.H. Xiao, S.J. Liu, X. Liu, S.X. Jiang, Application of ionic liquid in analytical chemistry, Chin. J. Anal. Chem. 4 (2005) 569-574.

    8. [8] Q.H. Cai, Y.W. Hou, S.Y. Shi, B. Lu, Y.K. Shan, A novel ionic liquid based on small size cation, Chin. Chem. Lett. 20 (2009) 62-65.[8] Q.H. Cai, Y.W. Hou, S.Y. Shi, B. Lu, Y.K. Shan, A novel ionic liquid based on small size cation, Chin. Chem. Lett. 20 (2009) 62-65.

    9. [9] V. Pino, A.M. Afonso, Surface-bonded ionic liquid stationary phases in high-performance liquid chromatography -a review, Anal. Chim. Acta 714 (2012) 20-37.[9] V. Pino, A.M. Afonso, Surface-bonded ionic liquid stationary phases in high-performance liquid chromatography -a review, Anal. Chim. Acta 714 (2012) 20-37.

    10. [10] H.D. Qiu, S.X. Jiang, X. Liu, N-Methylimidazolium anion-exchange stationary phase for high-performance liquid chromatography, J. Chromatogr. A 1103 (2006) 265-270.[10] H.D. Qiu, S.X. Jiang, X. Liu, N-Methylimidazolium anion-exchange stationary phase for high-performance liquid chromatography, J. Chromatogr. A 1103 (2006) 265-270.

    11. [11] H.D. Qiu, S.X. Jiang, X. Liu, L. Zhao, Novel imidazolium stationary phase for highperformance liquid chromatography, J. Chromatogr. A 1116 (2006) 46-50.[11] H.D. Qiu, S.X. Jiang, X. Liu, L. Zhao, Novel imidazolium stationary phase for highperformance liquid chromatography, J. Chromatogr. A 1116 (2006) 46-50.

    12. [12] T. Takeuchi, T. Kawasaki, L.W. Lim, Separation of inorganic anions on a pyridine stationary phase in ion chromatography, Anal. Sci. 26 (2010) 511-514.[12] T. Takeuchi, T. Kawasaki, L.W. Lim, Separation of inorganic anions on a pyridine stationary phase in ion chromatography, Anal. Sci. 26 (2010) 511-514.

    13. [13] L.M.L.A. Auler, C.R. Silva, K.E. Collins, C.H. Collins, New stationary phase for anionexchange chromatography, J. Chromatogr. A 1073 (2005) 147-153.[13] L.M.L.A. Auler, C.R. Silva, K.E. Collins, C.H. Collins, New stationary phase for anionexchange chromatography, J. Chromatogr. A 1073 (2005) 147-153.

    14. [14] S.J. Liu, F. Zhou, X.H. Xiao, et al., Surface confined ionic liquid -a new stationary phase for the separation of ephedrines in high-performance liquid chromatography, Chin. Chem. Lett. 15 (2004) 1060-1062.[14] S.J. Liu, F. Zhou, X.H. Xiao, et al., Surface confined ionic liquid -a new stationary phase for the separation of ephedrines in high-performance liquid chromatography, Chin. Chem. Lett. 15 (2004) 1060-1062.

    15. [15] H.D. Qiu, A.K. Mallik, M. Takafuji, H. Ihara, A facile and specific approach to new liquid chromatography adsorbents obtained by ionic self-assembly, Chem. Eur. J. 17 (2011) 7288-7297.[15] H.D. Qiu, A.K. Mallik, M. Takafuji, H. Ihara, A facile and specific approach to new liquid chromatography adsorbents obtained by ionic self-assembly, Chem. Eur. J. 17 (2011) 7288-7297.

    16. [16] H.D. Qiu, S.X. Jiang, M. Takafuji, H. Ihara, Polyanionic and polyzwitterionic azobenzene ionic liquid-functionalized silica materials and their chromatographic applications, Chem. Commun. 49 (2013) 2454-2456.[16] H.D. Qiu, S.X. Jiang, M. Takafuji, H. Ihara, Polyanionic and polyzwitterionic azobenzene ionic liquid-functionalized silica materials and their chromatographic applications, Chem. Commun. 49 (2013) 2454-2456.

    17. [17] Y.Q. Sun, A.M. Stalcup, Mobile phase effects on retention on a new butylimidazolium-based high-performance liquid chromatographic stationary phase, J. Chromatogr. A 1126 (2006) 276-282.[17] Y.Q. Sun, A.M. Stalcup, Mobile phase effects on retention on a new butylimidazolium-based high-performance liquid chromatographic stationary phase, J. Chromatogr. A 1126 (2006) 276-282.

    18. [18] A.J. Alpert, Electrostatic repulsion hydrophilic interaction chromatography for isocratic separation of charged solutes and selective isolation of phosphopeptides, Anal. Chem. 80 (2008) 62-76.[18] A.J. Alpert, Electrostatic repulsion hydrophilic interaction chromatography for isocratic separation of charged solutes and selective isolation of phosphopeptides, Anal. Chem. 80 (2008) 62-76.

    19. [19] K.L. Zhao, L. Yang, X.J. Wang, et al., Preparation of a novel dual-function strong cation exchange/hydrophobic interaction chromatography stationary phase for protein separation, Talanta 98 (2012) 86-94.[19] K.L. Zhao, L. Yang, X.J. Wang, et al., Preparation of a novel dual-function strong cation exchange/hydrophobic interaction chromatography stationary phase for protein separation, Talanta 98 (2012) 86-94.

  • 加载中
WeChat 扫一扫看文章
计量
  • PDF下载量:  0
  • 文章访问数:  1517
  • HTML全文浏览量:  45
文章相关
  • 发布日期:  2015-05-11
  • 收稿日期:  2014-12-24
  • 网络出版日期:  2015-04-15
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

/

返回文章

All Rights Reserved by the Chinese Chemical Society   中国化学会 版权所有 京ICP备05002797号

本系统由北京仁和汇智信息技术有限公司开发    技术支持: info@rhhz.net