引用本文:
Ru Feng, Jian Gong Shi, Xiao Wei Liu, Chun Tao Che, John H. K. Yeung, Yan Wang. Identification of the metabolites of biologically active xanthones isolated from Halenia elliptica D.Don by high performance liquid chromatography coupled to ion trap time-of-flight mass spectrometry[J]. Chinese Chemical Letters,
2011, 22(7): 839-842.
doi:
10.1016/j.cclet.2010.12.021
Citation: Ru Feng, Jian Gong Shi, Xiao Wei Liu, Chun Tao Che, John H. K. Yeung, Yan Wang. Identification of the metabolites of biologically active xanthones isolated from Halenia elliptica D.Don by high performance liquid chromatography coupled to ion trap time-of-flight mass spectrometry[J]. Chinese Chemical Letters, 2011, 22(7): 839-842. doi: 10.1016/j.cclet.2010.12.021
Citation: Ru Feng, Jian Gong Shi, Xiao Wei Liu, Chun Tao Che, John H. K. Yeung, Yan Wang. Identification of the metabolites of biologically active xanthones isolated from Halenia elliptica D.Don by high performance liquid chromatography coupled to ion trap time-of-flight mass spectrometry[J]. Chinese Chemical Letters, 2011, 22(7): 839-842. doi: 10.1016/j.cclet.2010.12.021
Identification of the metabolites of biologically active xanthones isolated from Halenia elliptica D.Don by high performance liquid chromatography coupled to ion trap time-of-flight mass spectrometry
摘要:
Metabolism study has been carried out on 1-hydroxy-2,3,5-trimethoxyxanthone (HM-1) and 1-hydroxy-2,3,4,7-tetramethoxyxanthone (HM-2), which are two biologically active ingredients isolated from the Tibetan herb, <Halenia elliptica D.Don., in rat liver microsomes in vitro. A method of high performance liquid chromatography coupled to ion trap time-of-flight mass spectrometry (LCMSn-ESI-IT-TOF) was applied to analyze metabolites of HM-1 and HM-2 on line, and five metabolites were identified containing 1,5-dihydroxy-2,3-dimethoxyxanthone (HM-5), 1,7-dihydroxy-2,3,4-trimethoxyxanthone (HM-9), 1,4, 7- trihydroxy-2,3-dimethoxyxanthone (HM-10), 1,4-dihydroxy-2,3,7-trimethoxyxanthone (HM-11) and 1,2-dihydroxy-3,4,7- trimethoxyxanthone (HM-12). Among these metabolites, HM-9, HM-11, and HM-12 were isomers mutually. The results indicated
that HM-1 and HM-2 occurred Phase I metabolic reaction of demethylation in rat microsomes in vitro.
English
Identification of the metabolites of biologically active xanthones isolated from Halenia elliptica D.Don by high performance liquid chromatography coupled to ion trap time-of-flight mass spectrometry
Abstract:
Metabolism study has been carried out on 1-hydroxy-2,3,5-trimethoxyxanthone (HM-1) and 1-hydroxy-2,3,4,7-tetramethoxyxanthone (HM-2), which are two biologically active ingredients isolated from the Tibetan herb, <Halenia elliptica D.Don., in rat liver microsomes in vitro. A method of high performance liquid chromatography coupled to ion trap time-of-flight mass spectrometry (LCMSn-ESI-IT-TOF) was applied to analyze metabolites of HM-1 and HM-2 on line, and five metabolites were identified containing 1,5-dihydroxy-2,3-dimethoxyxanthone (HM-5), 1,7-dihydroxy-2,3,4-trimethoxyxanthone (HM-9), 1,4, 7- trihydroxy-2,3-dimethoxyxanthone (HM-10), 1,4-dihydroxy-2,3,7-trimethoxyxanthone (HM-11) and 1,2-dihydroxy-3,4,7- trimethoxyxanthone (HM-12). Among these metabolites, HM-9, HM-11, and HM-12 were isomers mutually. The results indicated
that HM-1 and HM-2 occurred Phase I metabolic reaction of demethylation in rat microsomes in vitro.
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Key words:
- Halenia elliptica
- / Xanthone
- / Biotransformation
- / LCMSn-ESI-IT-TOF
- / Metabolite identification
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