引用本文:
Yu Yun Chen, Wei Ping Yang, Zhu Jun Zhang. Determination of metoprolol in rabbit blood using capillary electrophoresis with laser-induced fluorescence detection[J]. Chinese Chemical Letters,
2011, 22(3): 350-353.
doi:
10.1016/j.cclet.2010.10.025
Citation: Yu Yun Chen, Wei Ping Yang, Zhu Jun Zhang. Determination of metoprolol in rabbit blood using capillary electrophoresis with laser-induced fluorescence detection[J]. Chinese Chemical Letters, 2011, 22(3): 350-353. doi: 10.1016/j.cclet.2010.10.025
Citation: Yu Yun Chen, Wei Ping Yang, Zhu Jun Zhang. Determination of metoprolol in rabbit blood using capillary electrophoresis with laser-induced fluorescence detection[J]. Chinese Chemical Letters, 2011, 22(3): 350-353. doi: 10.1016/j.cclet.2010.10.025
Determination of metoprolol in rabbit blood using capillary electrophoresis with laser-induced fluorescence detection
摘要:
This work described a sensitive method for determination of metoprolol in rabbit plasma. The method involved purification by ultrafiltration, derivatization with fluorescein isothiocyanate, determination by capillary electrophoresis (CE) coupled with laser-induced fluorescence (LIF) detector. Other components in plasma including a variety of amino acids and proteins did not interfere with the determination of metoprolol in experimental condition. The assay had a wide range (2.0-500 ng/mL) of linearity and a detection limit of 0.8 ng/mL. The intra-and inter-day precisions were satisfactory with relative standard deviation (RSD) less than 10.0% and accuracy within 10.0%. This method was successfully applied to pharmacokinetic study of metoprolol in rabbit blood.
English
Determination of metoprolol in rabbit blood using capillary electrophoresis with laser-induced fluorescence detection
Abstract:
This work described a sensitive method for determination of metoprolol in rabbit plasma. The method involved purification by ultrafiltration, derivatization with fluorescein isothiocyanate, determination by capillary electrophoresis (CE) coupled with laser-induced fluorescence (LIF) detector. Other components in plasma including a variety of amino acids and proteins did not interfere with the determination of metoprolol in experimental condition. The assay had a wide range (2.0-500 ng/mL) of linearity and a detection limit of 0.8 ng/mL. The intra-and inter-day precisions were satisfactory with relative standard deviation (RSD) less than 10.0% and accuracy within 10.0%. This method was successfully applied to pharmacokinetic study of metoprolol in rabbit blood.
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Key words:
- Capillary electrophoresis
- / Metoprolol
- / Laser-induced fluorescence
- / Ultrafiltration
- / Pharmacokinetic
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