引用本文:
Saima Jadoon, Amir Waseem, Mohammad Yaqoob, Abdul Nabi. Flow injection spectrophotometric determination of vitamin E in Pharmaceuticals, milk powder and blood serum using potassium ferricyanide-Fe (Ⅲ) detection system[J]. Chinese Chemical Letters,
2010, 21(6): 712-715.
doi:
10.1016/j.cclet.2009.11.013
Citation: Saima Jadoon, Amir Waseem, Mohammad Yaqoob, Abdul Nabi. Flow injection spectrophotometric determination of vitamin E in Pharmaceuticals, milk powder and blood serum using potassium ferricyanide-Fe (Ⅲ) detection system[J]. Chinese Chemical Letters, 2010, 21(6): 712-715. doi: 10.1016/j.cclet.2009.11.013
Citation: Saima Jadoon, Amir Waseem, Mohammad Yaqoob, Abdul Nabi. Flow injection spectrophotometric determination of vitamin E in Pharmaceuticals, milk powder and blood serum using potassium ferricyanide-Fe (Ⅲ) detection system[J]. Chinese Chemical Letters, 2010, 21(6): 712-715. doi: 10.1016/j.cclet.2009.11.013
Flow injection spectrophotometric determination of vitamin E in Pharmaceuticals, milk powder and blood serum using potassium ferricyanide-Fe (Ⅲ) detection system
摘要:
A simple and sensitive flow injection spectrophotometric method is reported for the determination of vitamin E using potassium ferricyanide-Fe (Ⅲ) detection system. In the presence of vitamin E, Fe (Ⅲ/ferricyanide reduces. The in situ reduced ions are then reacted with unreduced portion of ferricyanide/Fe (Ⅲ to make soluble Prussian blue, which is monitored at absorption wavelength of 735 nm. Linear calibration graph was obtained in the concentration range of 0.1-40 μg mL-1. The relative standard deviations (n=4) were in the range of 1.1-3.6%, with limits of detection (3 s blank) of 0.04 μg mL-1. The proposed method allowed 12 injections h-1. The method is applied to determine vitamin E in Pharmaceuticals, infant milk and blood serum samples using hexane extraction with the recoveries in the range of 93 ±3 to 97.5 ±4%. The method is validated using certified reference materials SRM 968c for blood serum samples.
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关键词:
- Flow injection
- / Spectrophotometry
- / Vitamin E
- / Prussian blue
- / Fe (Ⅲ)
- / Ferricyanide
- / Pharmaceuticals
- / Blood serum
English
Flow injection spectrophotometric determination of vitamin E in Pharmaceuticals, milk powder and blood serum using potassium ferricyanide-Fe (Ⅲ) detection system
Abstract:
A simple and sensitive flow injection spectrophotometric method is reported for the determination of vitamin E using potassium ferricyanide-Fe (Ⅲ) detection system. In the presence of vitamin E, Fe (Ⅲ/ferricyanide reduces. The in situ reduced ions are then reacted with unreduced portion of ferricyanide/Fe (Ⅲ to make soluble Prussian blue, which is monitored at absorption wavelength of 735 nm. Linear calibration graph was obtained in the concentration range of 0.1-40 μg mL-1. The relative standard deviations (n=4) were in the range of 1.1-3.6%, with limits of detection (3 s blank) of 0.04 μg mL-1. The proposed method allowed 12 injections h-1. The method is applied to determine vitamin E in Pharmaceuticals, infant milk and blood serum samples using hexane extraction with the recoveries in the range of 93 ±3 to 97.5 ±4%. The method is validated using certified reference materials SRM 968c for blood serum samples.
-
Key words:
- Flow injection
- / Spectrophotometry
- / Vitamin E
- / Prussian blue
- / Fe (Ⅲ)
- / Ferricyanide
- / Pharmaceuticals
- / Blood serum
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