引用本文:
Min Chen, Guo Ping Ma, Li Sun. A new fluorescent particle prepared by chemical stabilized phycobilisome[J]. Chinese Chemical Letters,
2009, 20(6): 724-728.
doi:
10.1016/j.cclet.2008.12.042
Citation: Min Chen, Guo Ping Ma, Li Sun. A new fluorescent particle prepared by chemical stabilized phycobilisome[J]. Chinese Chemical Letters, 2009, 20(6): 724-728. doi: 10.1016/j.cclet.2008.12.042
Citation: Min Chen, Guo Ping Ma, Li Sun. A new fluorescent particle prepared by chemical stabilized phycobilisome[J]. Chinese Chemical Letters, 2009, 20(6): 724-728. doi: 10.1016/j.cclet.2008.12.042
A new fluorescent particle prepared by chemical stabilized phycobilisome
摘要:
Natural phycobilisomes (PBSs) were isolated and purified from a red macroalga, Polysiphonia urceolata, by multi-step of sucrose gradient centrifugation, and were chemically stabilized by small molecule cross-linker formaldehyde. The stabilized PBSs showed similar absorption and fluorescent properties at room temperature compared to natural PBSs and kept a steady F672/F580 value during more than 3 months of storage in 0.45 mol/L phosphate buffer (pH 6.8) or at low temperature at 77 K. The stabilized PBS migrated as a single band at mild PAGE and in 14-18 h of sucrose gradient centrifugation. All these characters indicated that the stabilized PBSs were stable, soluble, homogenous fluorescent particles with favorable spectroscopic features prepared under present conditions.
English
A new fluorescent particle prepared by chemical stabilized phycobilisome
Abstract:
Natural phycobilisomes (PBSs) were isolated and purified from a red macroalga, Polysiphonia urceolata, by multi-step of sucrose gradient centrifugation, and were chemically stabilized by small molecule cross-linker formaldehyde. The stabilized PBSs showed similar absorption and fluorescent properties at room temperature compared to natural PBSs and kept a steady F672/F580 value during more than 3 months of storage in 0.45 mol/L phosphate buffer (pH 6.8) or at low temperature at 77 K. The stabilized PBS migrated as a single band at mild PAGE and in 14-18 h of sucrose gradient centrifugation. All these characters indicated that the stabilized PBSs were stable, soluble, homogenous fluorescent particles with favorable spectroscopic features prepared under present conditions.
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Key words:
- Polysiphonia urceolata
- / PBSs
- / Chemical stabilization
- / Formaldehyde
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