Citation: Hai Yun ZHAI, Jun Mei WANG, Xiao Li YAO, Xue Cai TAN, Pei Xiang CAI, Zuan Guang CHEN. Analysis of Glutamic Acid in Cerebrospinal Fluid by Capillary Electrophoresis with High Frequency Conductivity Detection[J]. Chinese Chemical Letters, ;2005, 16(2): 225-228. shu

Analysis of Glutamic Acid in Cerebrospinal Fluid by Capillary Electrophoresis with High Frequency Conductivity Detection

1.
College of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275;
2.
Department of Chemistry, Medical College, Sun Yat-sen University, Guangzhou 510089;
3.
The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080

Received Date: 30 December 2003

Fund Project: This project was supported by the Guangdong Provincial Foundation of Natural Science (021808)

A rapid method to determine glutamic acid (Glu) in cerebrospinal fluid (CSF) by capillary electrophoresis with high frequency conductivity detection (contactless conductivity detection) was described. The CSF sample was pretreated with silver cation resin to remove high concentration of Cl^- ions in CSF. The separation was achieved in the buffer solution of 10 mmol/L Tris and 8 mmol/L boric acid at the separation voltage of 20.0 kV. Glu showed linear response in the range of 5.0×10^-6 to 6.0×10^-3 mol/L, the limit of detection was 1.0×10^-6 mol/L. The method was used for analysis Glu in CSF satisfactorily with a recovery of 97.8-98.8%.
- Capillary electrophoresis,
- high frequency conductivity detection,
- contactless conductivity detection,
- glutamic acid,
- cerebrospinal fluid.
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