Citation: Shan Guangcheng, Pan Yumei, Chen Zhijuan, Gu Bei, Shen Jian, Wu shishan, Chen Qiang. Preparation of Zwitterionic Polymer and Its Effect on Enzyme Activity[J]. Chemistry, ;2017, 80(7): 688-691. shu

Preparation of Zwitterionic Polymer and Its Effect on Enzyme Activity

Shan Guangcheng ¹ ,
Pan Yumei ¹ ,
Chen Zhijuan ¹ ,
Gu Bei ¹ ,
Shen Jian ^1,3 ,
Wu shishan ^1,3 ,
Chen Qiang ^1,2,3,,

1.
School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023
2.
High-Tech Research Institute of Nanjing University, Changzhou 213164
3.
Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, Nanjing 210093

Corresponding author: Chen Qiang, chem100@nju.edu.cn
Received Date: 29 December 2016
Accepted Date: 20 February 2017

Figures(4)

The carboxyl betaine zwitterionic polymer (PCBMA) was synthesized by free radical polymerization, the biocompatible zwitterionic carboxybetaine methacrylate (CBMA) as monomer, ammonium persulfate as initiator and sodium sulfite as a reductant. The results of ¹H NMR and GPC indicated that the polymer has expected structure. As a model enzyme, the glucose oxidase (GOD) was prepared with and without PCBMA to investigate the relationship between the activity of GOD and time under different temperatures. The results showed that the activity concentration of GOD with PCBMA at 65℃ after 12h was 103% of that without PCBMA for 1h, whereas the GOD activity of control after 12h was only 35% of that without PCBMA for 1h. The carboxyl betaine zwitterionic polymer could effectively maintain the enzyme activity, and this effect was more evident as the temperature increases.
- Zwitterionic polymer,
- Glucose oxidase,
- Activity
1. [1]
  M Claude, R Lupi, G Bouchaud et al. Food Chem., 2016, 203:136~144.
2. [2]
  W G Dai, L C Dong. Int. J. Pharm., 2007, 336(1):58~66.
3. [3]
  S Y Jiang, Z Q Cao. Adv. Mater., 2010, 22(9):920~932.
4. [4]
  Z Zhang, H Vaisocherova, G Cheng et al. Biomacromolecules, 2008, 9(10):2686~2692.
5. [5]
  J Ladd, Z Zhang, S Chen et al. Biomacromolecules, 2008, 9(5):1357~1361.
6. [6]
7. [7]
  M C Parrott, J M Desimone. Nat. Chem., 2011, 4(1):13~16.
8. [8]
  Y Y Jiang, H X Lu, F Chen et al. Biomacromolecules, 2016,17(3):807~808.
9. [9]
  A J Keefe, S Y Jiang. Nat. Chem., 2011, 4(1):59~63.
10. [10]
  S Kudaibergenov, W Jaeger, A Laschewsky. Polymeric betaines:synthesis, characterization, and application[M]//Supramolecular polymers polymeric betains oligomers. Springer Berlin Heidelberg, 2006:157~224.
11. [11]
  S Y Liu, S Y Jiang. Nano Today, 2016, 11:285~291.
12. [12]
  Q Shao, S Y Jiang. Adv. Mater., 2015, 27(1):15~26.
13. [13]
  M C Sin, P T Lou, C H Cho et al. Colloid. Surf. B, 2015, 127:54~64.
14. [14]
15. [15]
16. [16]
  Z Zhang, T Chao, S Y Jiang et al. Langmuir, 2006, 22(24):10072~10077.
17. [17]
1. [1]
  Bin SUN , Heyan JIANG . Glucose-modified bis-Schiff bases: Synthesis and bio-activities in Alzheimer′s disease therapy. Chinese Journal of Inorganic Chemistry, 2025, 41(7): 1338-1350. doi: 10.11862/CJIC.20240428
2. [2]
  Lihua HUANG , Jian HUA . Denitration performance of HoCeMn/TiO₂ catalysts prepared by co-precipitation and impregnation methods. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 629-645. doi: 10.11862/CJIC.20230315
3. [3]
  Zilin Hu , Yaoshen Niu , Xiaohui Rong , Yongsheng Hu . Suppression of Voltage Decay through Ni³⁺ Barrier in Anionic-Redox Active Cathode for Na-Ion Batteries. Acta Physico-Chimica Sinica, 2024, 40(6): 2306005-0. doi: 10.3866/PKU.WHXB202306005
4. [4]
  Quanliang Chen , Zhaohui Zhou . Research on the Active Site of Nitrogenase over Fifty Years. University Chemistry, 2024, 39(7): 287-293. doi: 10.3866/PKU.DXHX202310133
5. [5]
  Chunmei GUO , Weihan YIN , Jingyi SHI , Jianhang ZHAO , Ying CHEN , Quli FAN . Facile construction and peroxidase-like activity of single-atom platinum nanozyme. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1633-1639. doi: 10.11862/CJIC.20240162
6. [6]
  Haitang WANG , Yanni LING , Xiaqing MA , Yuxin CHEN , Rui ZHANG , Keyi WANG , Ying ZHANG , Wenmin WANG . Construction, crystal structures, and biological activities of two Ln^Ⅲ₃ complexes. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1474-1482. doi: 10.11862/CJIC.20240188
7. [7]
  Jingjing QING , Fan HE , Zhihui LIU , Shuaipeng HOU , Ya LIU , Yifan JIANG , Mengting TAN , Lifang HE , Fuxing ZHANG , Xiaoming ZHU . Synthesis, structure, and anticancer activity of two complexes of dimethylglyoxime organotin. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1301-1308. doi: 10.11862/CJIC.20240003
8. [8]
  Jiayu Gu , Siqi Wang , Jun Ling . Kinetics of Living Copolymerization: A Brief Discussion. University Chemistry, 2025, 40(4): 100-107. doi: 10.12461/PKU.DXHX202406012
9. [9]
  Rong Tian , Yadi Yang , Naihao Lu . Comprehensive Experimental Design of Undergraduate Students Based on Interdisciplinarity: Study on the Effect of Quercetin on Chlorination Activity of Myeloperoxidase. University Chemistry, 2024, 39(8): 247-254. doi: 10.3866/PKU.DXHX202312064
10. [10]
  Xinting XIONG , Zhiqiang XIONG , Panlei XIAO , Xuliang NIE , Xiuying SONG , Xiuguang YI . Synthesis, crystal structures, Hirshfeld surface analysis, and antifungal activity of two complexes Na(Ⅰ)/Cd(Ⅱ) assembled by 5-bromo-2-hydroxybenzoic acid ligands. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1661-1670. doi: 10.11862/CJIC.20240145
11. [11]
  Linjie ZHU , Xufeng LIU . Synthesis, characterization and electrocatalytic hydrogen evolution of two di-iron complexes containing a phosphine ligand with a pendant amine. Chinese Journal of Inorganic Chemistry, 2025, 41(5): 939-947. doi: 10.11862/CJIC.20240416
12. [12]
  Yuanqing Wang , Yusong Pan , Hongwu Zhu , Yanlei Xiang , Rong Han , Run Huang , Chao Du , Chengling Pan . Enhanced Catalytic Activity of Bi₂WO₆ for Organic Pollutants Degradation under the Synergism between Advanced Oxidative Processes and Visible Light Irradiation. Acta Physico-Chimica Sinica, 2024, 40(4): 2304050-0. doi: 10.3866/PKU.WHXB202304050
13. [13]
  Lifang HE , Wenjie TANG , Yaoze LUO , Mingsheng LIANG , Jianxin TANG , Yuxuan WU , Fuxing ZHANG , Xiaoming ZHU . Synthesis, structure, and anticancer activity of two dialkyltin complexes constructed based on 2, 2′-bipyridin-6, 6′-dicarboxylic acid. Chinese Journal of Inorganic Chemistry, 2025, 41(8): 1601-1609. doi: 10.11862/CJIC.20250012
14. [14]
  Changqing MIAO , Fengjiao CHEN , Wenyu LI , Shujie WEI , Yuqing YAO , Keyi WANG , Ni WANG , Xiaoyan XIN , Ming FANG . Crystal structures, DNA action, and antibacterial activities of three tetranuclear lanthanide-based complexes. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2455-2465. doi: 10.11862/CJIC.20240192
15. [15]
  Jing WU , Puzhen HUI , Huilin ZHENG , Pingchuan YUAN , Chunfei WANG , Hui WANG , Xiaoxia GU . Synthesis, crystal structures, and antitumor activities of transition metal complexes incorporating a naphthol-aldehyde Schiff base ligand. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2422-2428. doi: 10.11862/CJIC.20240278
16. [16]
  Xiaotong LU , Pan ZHANG , Zijie ZHAO , Lei HUANG , Hongwei ZUO , Lili LIANG . Antitumor and antibacterial activities of pyridyl Schiff base indium and dysprosium complexes. Chinese Journal of Inorganic Chemistry, 2025, 41(8): 1523-1532. doi: 10.11862/CJIC.20250073
17. [17]
  Ruiying WANG , Hui WANG , Fenglan CHAI , Zhinan ZUO , Benlai WU . Three-dimensional homochiral Eu(Ⅲ) coordination polymer and its amino acid configuration recognition. Chinese Journal of Inorganic Chemistry, 2025, 41(5): 877-884. doi: 10.11862/CJIC.20250052
18. [18]
  Jianan Hong , Chenyu Xu , Yan Liu , Changqi Li , Menglin Wang , Yanwei Zhang . Decoding the interfacial competition between hydrogen evolution and CO₂ reduction via edge-active-site modulation in photothermal catalysis. Acta Physico-Chimica Sinica, 2025, 41(9): 100099-0. doi: 10.1016/j.actphy.2025.100099
19. [19]
  Wentao Xu , Xuyan Mo , Yang Zhou , Zuxian Weng , Kunling Mo , Yanhua Wu , Xinlin Jiang , Dan Li , Tangqi Lan , Huan Wen , Fuqin Zheng , Youjun Fan , Wei Chen . Bimetal Leaching Induced Reconstruction of Water Oxidation Electrocatalyst for Enhanced Activity and Stability. Acta Physico-Chimica Sinica, 2024, 40(8): 2308003-0. doi: 10.3866/PKU.WHXB202308003
20. [20]
  Jichao XU , Ming HU , Xichang CHEN , Chunhui WANG , Leichen WANG , Lingyi ZHOU , Xing HE , Xiamin CHENG , Su JING . Construction and hydrogen peroxide-activated chemodynamic activity of ferrocene?benzoselenadiazole conjugate. Chinese Journal of Inorganic Chemistry, 2025, 41(8): 1495-1504. doi: 10.11862/CJIC.20250144

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