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Citation: Chen Ruichuan, Lu Fang, Li Donghui, Zhang Changgong, Wan Zhen. Application of Functional Electropolymerized m-Phenylene Diamine Membrane in Immunosensor Analysis[J]. Chinese Journal of Applied Chemistry, ;1998, 15(6): 25-28. shu

Application of Functional Electropolymerized m-Phenylene Diamine Membrane in Immunosensor Analysis

1.
Biochemistry Laboratory of Cancer Research Center, Xiamen University, Xiamen 361005;
2.
Department of Chemistry, Xiamen University, Xiamen 361005

Received Date: 23 February 1998
Revised Date: 9 September 1998

An electropolymerization method of m-Phenylene diamine membrane, polyphenylene diamine(PMPD) on the surface of graphite electrode is described. To identify the quality of the membrane, a rapid and sensitive method has been established based on the reaction of a horseradish peroxidase(HRP) labeled PMPD with orthophenylene diamine. An antiHBsAg and an anti-MIgG immunoelectrodes were prepared for detecting hepatitis B surface antigen(HBsAg) and mouse immunoglobin G(MIgG). The optimum membrane preparation conditions are polymerization:0.12 mol/L MPD solution in 1.2 mol/L H₂SO₄ for 20 min under 2.5 V, activation:with 3% glutiraldehyde for 4 h. The prepared HRP labeled electrode and immunoelectrodes possessed good repetition. Catibration graphs were linear for HBsAg in ranges from 0.1~3.2μg/mL and 0.1~2.0μg/mL for MIgG/mL.
- immunosensor,
- chemically modified electrode,
- poly-m-Phenylene diamine
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