Citation: Yin Ling, Wang Anna, Zhao Meng, Sha Jingquan, Bai Yongxiang, Shi Haibin. Preparation of A Novel Smart Gold Nanorod Probe and Its Application in Cancer Cell Imaging[J]. Chemistry, ;2019, 82(1): 44-50. shu

Preparation of A Novel Smart Gold Nanorod Probe and Its Application in Cancer Cell Imaging

Yin Ling ^2,3 ,
Wang Anna ¹ ,
Zhao Meng ¹ ,
Sha Jingquan ³ ,
Bai Yongxiang ⁴ ,
Shi Haibin ^1,,

1.
State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection and Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Soochow University, Suzhou 215123
2.
Key Laboratory of Organic Synthesis of Jiangsu Province, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123
3.
Department of Chemistry and Chemical Engineering, Jining University, Qufu 273155
4.
School of Biology and Basic Medical Sciences, Soochow University, Suzhou 215123

Corresponding author: Shi Haibin, bi@uda.edu.cn
Received Date: 8 August 2018
Accepted Date: 16 October 2018

Figures(5)

Developing a smart fluorescent probe which can specifically and sensitively detect matrix metalloproteinases-2 (MMP-2) is vital for the early accurate diagnosis and treatment of cancers. In this study, fluorescein isothiocyanate (FITC) was firstly conjugated to the peptide substrate GPLGVRGY by using copper (I)-catalyzed "click" reaction, and subsequently reacted with gold nanorods decorated with SH-PEG-COOH under the catalysis of EDC and Sulfo-NHS through amidation reaction to afford a novel fluorescence resonance energy transfer (FRET)-based probe GNR@FITC for specific detection of MMP-2. In vitro enzyme detection experiments, MTT assays and fluorescence imaging results showed that the probe shows good specificity and high sensitivity for the detection of MMP-2-overexpressed 4T1 cells, which may have great potential for clinical application in early diagnosis of breast cancer.
- Matrix metalloprotease-2(MMP-2),
- Gold nanorods,
- Fluorescence resonance energy transfer(FRET),
- Molecular probe,
- Fluorescent imaging
1. [1]
  M Egeblad, Z Werb. Nat. Rev. Cancer, 2002, 2:161~174.
2. [2]
  S Lee, J Xie, X Chen. Chem. Rev., 2010, 110(5):3087~3111.
3. [3]
  H Hanaoka, T Mukai, S Habashita et al. Nucl. Med. Biol., 2007, 34(5):503~510.
4. [4]
  J Gallo, N Kamaly, I Lavdas et al. Angew. Chem. Int. Ed., 2014, 53(36):9550~9554.
5. [5]
  K C Black, W J Akers, G Sudlow et al. Nanoscale, 2015, 7(2):440~444.
6. [6]
  Y Tang, Z Wu, C H Zhang et al. Chem. Commun., 2016, 52(18):3631~3634.
7. [7]
8. [8]
  N Halas. MRS Bull., 2005, 30(5):362~367.
9. [9]
  F Sonvico, C Dubernet, P Colombo et al. Curr. Pharm. Des., 2005, 11(16):2095~2105.
10. [10]
  Y Xia, N J Halas. MRS Bull., 2005, 30(5):338~348.
11. [11]
  K T Yong, Y Sahoo, M T Swihart et al. Colloid. Surf. A, 2006, 290(1):89~105.
12. [12]
  X J Cheng, R Sun, L Yin et al. Adv. Mater., 2017, 29(6):DOI:10.1002/adma.201604894.
13. [13]
  T Niidome, M Yamagata, Y Okamoto et al. J. Control. Release, 2006, 114(3):343~347.
14. [14]
  T B Huff, M N Hansen, Y Zhao et al. Langmuir, 2007, 23(4):1596~1599.
15. [15]
  Y Akiyama, T Mori, Y Katayama et al. J. Control. Release, 2009, 139(1):81~84.
16. [16]
  S Pierrat, I Zins, A Breivogel et al. Nano Lett., 2007, 7(2):259~263.
17. [17]
  E Dulkeith, A C Morteani, T Niedereichholz et al. Phys. Rev. Lett., 2002, 89(20):203002.
18. [18]
  L M Demers, C A Mirkin, R C Mucic et al. Anal. Chem., 2000, 72(22):5535~5541.
19. [19]
  F Blasi, P Carmeliet. Nat. Rev. Mol. Cell Biol., 2002, 3(12):932~943.
20. [20]
  J Qian, L Jiang, F H Cai et al. Biomaterials, 2011, 32(6):1601~1610.
1. [1]
  Siyi ZHONG , Xiaowen LIN , Jiaxin LIU , Ruyi WANG , Tao LIANG , Zhengfeng DENG , Ao ZHONG , Cuiping HAN . Targeting imaging and detection of ovarian cancer cells based on fluorescent magnetic carbon dots. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1483-1490. doi: 10.11862/CJIC.20240093
2. [2]
  Hong LI , Xiaoying DING , Cihang LIU , Jinghan ZHANG , Yanying RAO . Detection of iron and copper ions based on gold nanorod etching colorimetry. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 953-962. doi: 10.11862/CJIC.20230370
3. [3]
  Hongpeng He , Mengmeng Zhang , Mengjiao Hao , Wei Du , Haibing Xia . Synthesis of Different Aspect-Ratios of Fixed Width Gold Nanorods. Acta Physico-Chimica Sinica, 2024, 40(5): 2304043-0. doi: 10.3866/PKU.WHXB202304043
4. [4]
  Jichao XU , Ming HU , Xichang CHEN , Chunhui WANG , Leichen WANG , Lingyi ZHOU , Xing HE , Xiamin CHENG , Su JING . Construction and hydrogen peroxide-activated chemodynamic activity of ferrocene?benzoselenadiazole conjugate. Chinese Journal of Inorganic Chemistry, 2025, 41(8): 1495-1504. doi: 10.11862/CJIC.20250144
5. [5]
  Jinlong YAN , Weina WU , Yuan WANG . A simple Schiff base probe for the fluorescent turn-on detection of hypochlorite and its biological imaging application. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1653-1660. doi: 10.11862/CJIC.20240154
6. [6]
  Yanxi LIU , Mengjia XU , Haonan CHEN , Quan LIU , Yuming ZHANG . A fluorescent-colorimetric probe for peroxynitrite-anion-imaging in living cells. Chinese Journal of Inorganic Chemistry, 2025, 41(6): 1112-1122. doi: 10.11862/CJIC.20240423
7. [7]
  Jun LUO , Baoshu LIU , Yunchang ZHANG , Bingkai WANG , Beibei GUO , Lan SHE , Tianheng CHEN . Europium(Ⅲ) metal-organic framework as a fluorescent probe for selectively and sensitively sensing Pb²⁺ in aqueous solution. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2438-2444. doi: 10.11862/CJIC.20240240
8. [8]
  Yingpeng ZHANG , Xingxing LI , Yunshang YANG , Zhidong TENG . A pyrazole-based turn-off fluorescent probe for visual detection of hydrazine. Chinese Journal of Inorganic Chemistry, 2025, 41(7): 1301-1308. doi: 10.11862/CJIC.20250064
9. [9]
  Yuting DU , Jing YUAN , Peiyao DENG . Synthesis and application of a fluorescent probe for the detection of reduced glutathione. Chinese Journal of Inorganic Chemistry, 2025, 41(7): 1331-1337. doi: 10.11862/CJIC.20240461
10. [10]
  Yue WANG , Zhizhi GU , Jingyi DONG , Jie ZHU , Cunguang LIU , Guohan LI , Meichen LU , Jian HAN , Shengnan CAO , Wei WANG . Effects of kelp-derived carbon dots on embryonic development of zebrafish. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1209-1217. doi: 10.11862/CJIC.20230423
11. [11]
  Jiakun BAI , Ting XU , Lu ZHANG , Jiang PENG , Yuqiang LI , Junhui JIA . A red-emitting fluorescent probe with a large Stokes shift for selective detection of hypochlorous acid. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1095-1104. doi: 10.11862/CJIC.20240002
12. [12]
  Yu Dai , Xueting Sun , Haoyu Wu , Naizhu Li , Guoe Cheng , Xiaojin Zhang , Fan Xia . Determination of the Michaelis Constant for Gold Nanozyme-Catalyzed Decomposition of Hydrogen Peroxide. University Chemistry, 2025, 40(5): 351-356. doi: 10.12461/PKU.DXHX202407052
13. [13]
  Zijuan LI , Xuan LÜ , Jiaojiao CHEN , Haiyang ZHAO , Shuo SUN , Zhiwu ZHANG , Jianlong ZHANG , Yanling MA , Jie LI , Zixian FENG , Jiahui LIU . Synthesis of visual fluorescence emission CdSe nanocrystals based on ligand regulation. Chinese Journal of Inorganic Chemistry, 2025, 41(2): 308-320. doi: 10.11862/CJIC.20240138
14. [14]
  Benhua Wang , Chaoyi Yao , Yiming Li , Qing Liu , Minhuan Lan , Guipeng Yu , Yiming Luo , Xiangzhi Song . 一种基于香豆素氟离子荧光探针的合成、表征及性能测试——“科研反哺教学”在有机化学综合实验教学中的探索与实践. University Chemistry, 2025, 40(6): 201-209. doi: 10.12461/PKU.DXHX202408070
15. [15]
  Xinyi Hong , Tailing Xue , Zhou Xu , Enrong Xie , Mingkai Wu , Qingqing Wang , Lina Wu . Non-Site-Specific Fluorescent Labeling of Proteins as a Chemical Biology Experiment. University Chemistry, 2024, 39(4): 351-360. doi: 10.3866/PKU.DXHX202310010
16. [16]
  Feng Lu , Tao Wang , Qi Wang . Preparation and Characterization of Water-Soluble Silver Nanoclusters: A New Design and Teaching Practice in Materials Chemistry Experiment. University Chemistry, 2025, 40(4): 375-381. doi: 10.12461/PKU.DXHX202406005
17. [17]
  Qin Li , Huihui Zhang , Huajun Gu , Yuanyuan Cui , Ruihua Gao , Wei-Lin Dai . In situ Growth of Cd_0.5Zn_0.5S Nanorods on Ti₃C₂ MXene Nanosheet for Efficient Visible-Light-Driven Photocatalytic Hydrogen Evolution. Acta Physico-Chimica Sinica, 2025, 41(4): 2402016-0. doi: 10.3866/PKU.WHXB202402016
18. [18]
  Peiran ZHAO , Yuqian LIU , Cheng HE , Chunying DUAN . A functionalized Eu³⁺ metal-organic framework for selective fluorescent detection of pyrene. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 713-724. doi: 10.11862/CJIC.20230355
19. [19]
  Yan ZHAO , Xiaokang JIANG , Zhonghui LI , Jiaxu WANG , Hengwei ZHOU , Hai GUO . Preparation and fluorescence properties of Eu³⁺-doped CaLaGaO₄ red-emitting phosphors. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1861-1868. doi: 10.11862/CJIC.20240242
20. [20]
  Jinghan ZHANG , Guanying CHEN . Progress in the application of rare-earth-doped upconversion nanoprobes in biological detection. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2335-2355. doi: 10.11862/CJIC.20240249

Get Citation

PDF

XML

Metrics

PDF Downloads(11)
Abstract views(533)
HTML views(183)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142