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Citation: Dong Xu, Guo Hua, Lv Chunlin, Shen Gangyi. Determination of Glutathione Content Based on Graphene Nanoenzyme Effect[J]. Chemistry, ;2019, 82(7): 659-662. shu

Determination of Glutathione Content Based on Graphene Nanoenzyme Effect

Key Laboratory of Ethnomedicine(Minzu University of China), Ministry of Education, School of Pharmacy, Minzu University of China, Beijing 100081

Corresponding author: Shen Gangyi, shengy@muc.edu.cn
Received Date: 5 March 2019
Accepted Date: 9 April 2019

Figures(6)

A new method for the determination of glutathione content was established by using the nanoenzyme effect of carboxylated graphene oxide (GO-COOH). The reaction time, pH, and H₂O₂ concentration were optimized. Under optimal conditions, the linear range of glutathione (GSH) standards was 1~50 μmol/L. The method was applied to the determination of practical sample, and verified by high performance liquid chromatography. The results showed that the content of glutathione tablets was easily and accurately measured with the relative error of 1.8%, indicating that the method is simple and intuitive, and can accurately measure the content of glutathione tablet.
- Glutathione,
- Assay,
- Nanoparticle enzyme,
- Carboxylated graphene oxide
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