Citation: Hai Fei ZHANG, Bu Xing HAN, Jie LU, Guan Ying YANG, Hai Ke YAN. Effect of Dissolved CO₂ on Protein Solubilization in Reverse Micelle[J]. Chinese Chemical Letters, ;1999, 10(4): 331-332. shu

Effect of Dissolved CO₂ on Protein Solubilization in Reverse Micelle

Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080

Received Date: 20 August 1998

Fund Project: The authors are grateful to the National Natural Science Foundation of China for the financial support (29725308,29633020).

Effect of dissolved CO₂ on bovine serum albumin (BSA) solubilization in the reverse micelles of sodium bis (2-ethylhexyl) sulfosuccinate (AOT) in 2,2,4-trimethylpentane (iso-octane) has been studied at 308.2 K. It was found that BSA can be precipitated completely by CO₂ while the AOT and water remain in the iso-octane continuous phase. This opens up a possibility for recovery of pure protein solids directly from reverse micellar solutions.
- Compressed CO₂,
- protein,
- precipitation,
- reverse micelle
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