Citation: Xu Tianqi, Yin Fangfei, Zhang Xiaohua, Zuo Xiaolei. Sequencing-Free Detection of Single Nucleotide Polymorphism[J]. Chemistry, ;2020, 83(6): 497-507. shu

Sequencing-Free Detection of Single Nucleotide Polymorphism

Xu Tianqi ¹ ,
Yin Fangfei ^2,3 ,
Zhang Xiaohua ^1,, ,
Zuo Xiaolei ^1,,

1.
Institute of Molecular Medicine, School of Medicine, Shanghai Jiaotong University, and Department of Neurosurgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127
2.
Division of Physical Biology, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, 201800
3.
University of Chinese Academy of Sciences, Beijing, 100049

Corresponding author: Zhang Xiaohua, zxh1969@aliyun.com Zuo Xiaolei, zuoxiaolei@sjtu.edu.cn
Received Date: 9 January 2020
Accepted Date: 2 March 2020

Figures(5)

Single nucleotide polymorphism (SNP) is vastly prevalent in genome mutations, which has been proved to be highly associated with various types of diseases. While sequencing detection plays a vital part in SNP detection, its dependence on equipment and time consumption confines the clinical application. This review focuses on sequencing-free detection of SNP. Thermodynamic aspects are first discussed, followed by major detection strategies:hybridization-based detection, strand displacement reaction, and enzyme-mediated detection. Three categories of detection methods are then elucidated. In three-dimensional homogeneous detection, signal switch strategies such as fluorophore switch, enzyme recognition switch, and field effect switch are elaborated. Three-dimentional detection in situ provides the location of SNP in addition to its presence, showing advantages in SNP detection in heterogeneous cells. In two-dimensional interface detection, despite the compromised reaction rate and hybridization efficiency, the nature of chip detection facilitates multiplexed detection as well as minimized interferences. Corrected chips like DNA tetrahedron-structured probes (TSP) show optimized detection sensitivity and specificity. Setbacks and further research directions in the field are also discussed.
- Single nucleotide polymorphism,
- Sequencing-free detection,
- Homogenous detection,
- In situ detection,
- Interface detection
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