Citation: Ya Shan HAN, Yun Qing DAI. SIMULTANEOUS DETERMINATION OF FAT SOLUBLE VITAMINS A, D₃, E AND K₁ IN FORTIFIED MILK POWDERS BY HPLC[J]. Chinese Chemical Letters, ;1991, 3(8): 649-652. shu

SIMULTANEOUS DETERMINATION OF FAT SOLUBLE VITAMINS A, D₃, E AND K₁ IN FORTIFIED MILK POWDERS BY HPLC

Department of Food science Beijing Agricultural University Beijing 100094

Received Date: 25 March 1991

A high performance liquid chromatographic method for the simultaneous determination of four fat soluble vitamins: retinol (vitamin A), cholecalciferol(vitamin D), tocopherol(vitamin E) and phylloquinone(vitamin K₁) in fortified milk powders and egg yolk has been developed. The method requires enzymatic hydrolysis of lipid component of the sample as a pretreatment. Several factors which influence the enzymatic hydrolysis were studied-Separation was achieved using μ-Bondapak C-18 column(3.9×300mm), 98% methanol as mobile phase, a double channel detection was selected; vitamins D₃ E₁ K₁ were detected by UV spectrophotometry (265 nm) first, then vitamin A by fluorometry (EX 325nm, EM 480nm). The retention times of vitamin A₁ D₃, E and K₁ 4.87, 9.00, 10.58 and 15.45 min respectively. Detection limit were 0.64, 0.25, 0.50 and 0.07 ng; and the recoveries were 90.5%~103.6%, 90.0%~95.6%, 91.7%~98.8%, 91.5%~98.6%, respectively. The vitamins A, D₃, E, K₁ contents in foods were determined satisfactorily.
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