Citation: Liu Yanhua, Huang Dengfa, Zhu Jinwen, Cui Zhenggang, Wang Feng. Enzymatic Synthesis of L-Phenylalanine Polypeptide[J]. Chemistry, ;2018, 81(4): 361-365, 383. shu

Enzymatic Synthesis of L-Phenylalanine Polypeptide

School of Chemical and Material Engineering, Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, Jiangnan University, Wuxi 214122

Corresponding author: Wang Feng, fwang@jiangnan.edu.cn
Received Date: 23 September 2017
Accepted Date: 18 January 2018

Figures(11)

Artificial polymers of amino acids, polypeptides, show the advantages of biocompatibility and biodegradability. In this study, the oligo-L-phenylalanine was prepared by the polymerization reaction of bromelain from L-phenylalanine methyl ester in the non-aqueous media under mild conditions. The structure and polymerization degree of the oligo-L-phenylalanine were elucidated by FT-IR, ¹H-NMR, MS and MALDI-TOF. The optimum polymerization conditions were evaluated through single-factor approach. The highest value of yield, 35.67%, could be achieved when 0.43g L-phenylalanine methyl ester hydrochloride in 15% DMSO phosphate buffer (pH=8.0) was polymerized by 2.44U bromelain at 40℃ for 3 hours.
- L-phenylalanine methyl ester hydrochloride,
- Enzymatic Catalysis,
- Polymerization,
- Characterization
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