Citation: Qi Danping, Lin Jiangli, Gao Min, Lu Jishuai. Pectinase Immobilization Using HPD-750 Macroporous Resin as Carrier Material[J]. Chemistry, ;2020, 83(2): 161-166. shu

Pectinase Immobilization Using HPD-750 Macroporous Resin as Carrier Material

College of Chemistry and Chemical Engineering, Xinjiang University, Urumchi, 830046

Corresponding author: Lin Jiangli, 736058563@qq.com
Received Date: 2 August 2019
Accepted Date: 30 November 2019

Figures(9)

HPD-750 resin is a medium-polar macroporous adsorption resin with good biocompatibility, stable mechanical properties and large specific surface area, which can be used for immobilizing enzyme. In this paper, pectinase was immobilized by HPD-750 macroporous resin, and the effects of various factors on the immobilized enzyme were studied. The optimized immobilization parameters are:pH 4.0, the immobilization temperature 45℃, the immobilization time 4h, and the amount of enzyme added 0.16g/mL, Under these conditions, the immobilized enzyme activity can reach 5146U/mg. Compared with free pectinase, the immobilized enzyme was found to exhibit better stability of pH and thermo. Additionally, the immobilized pectinase had remarkable reusability and storage stability, the enzyme activity remained more than 80% after 10 cycles of use; and after storage at 4℃ for 25 days, the enzyme activity remained more than 60%. Compared with immobilized enzyme prepared D311 macroporous resin, polyacrylamide and sodium alginate microspheres, the HPD-750 immobilized enzyme has better activity, operational stability, mechanical stability and storage stability. Hence that the HPD-750 macroporous resin could be used as a good carrier material for immobilized enzymes.
- HPD-750 macroporous resin,
- Carrier material,
- Pectinase,
- Immobilized
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沈阳化工大学材料科学与工程学院沈阳 110142