Citation: Liu Jia, Zhang Lei, Shen Gangyi. Research Advance on Immobilized Enzyme Using Graphene as Carrier[J]. Chemistry, ;2017, 80(1): 41-46. shu

Research Advance on Immobilized Enzyme Using Graphene as Carrier

Key Laboratory of Chinese Minority Traditional Medicine, State Ethnic Affairs Commission and Ministry of Education of China, Institute of Chinese Minority Traditional Medicine, Minzu University of China, Beijing 100081

Corresponding author: Shen Gangyi, sgy@iccas.ac.cn
Received Date: 15 June 2016
Accepted Date: 31 July 2016

Figures(7)

The immobilized enzyme has been widely applied to the food and medicine industrial production, and micro biochemical detection field for its advantages of high efficiency catalysis, recyclability and easy separation. The performance of immobilized enzyme is closely related to the carrier materials. As a new type of nano materials, graphene has excellent physical and chemical properties, which is an ideal carrier for the immobilization of enzyme. This article focused on the technology of immobilized enzyme using graphene as carrier for the past few years. The immobilization methods including physical adsorption, chemical bonding and encapsulation were particularly discussed. The recent applications of immobilized enzyme in the field of enzyme micro-reaction and biosensor were reviewed. The prospects of its future development were also outlined.
- Immobilized enzyme,
- Graphene,
- Preparaiton,
- Application
1. [1]
2. [2]
3. [3]
  J Iqbal, S Iqba, C E Müller. Analyst, 2013, 138:3104-3116.
4. [4]
  J M Nelson, E G Griffin. J. Am. Chem. Soc., 1916, 38:1109-1115.
5. [5]
6. [6]
  Y Li, X Q Xu, C H Deng et al. J. Proteome Res., 2007, 6:3849-3855.
7. [7]
  L Wang, R Xu, Y Chen et al. J. Mol. Catal. B-Enzym., 2011, 69:120-126.
8. [8]
  T T Trupti, K Kikuo, R Pankaj et al. Langmuir, 2015, 31:13054-13061.
9. [9]
  S J Guo, H Li, J Liu et al. Appl. Mater. Interf., 2015, 7:20937-20944.
10. [10]
  G L Zhang, M L Yang, D Q Cai et al. Appl. Mater. Interf., 2014, 6:8042-8047.
11. [11]
  X Y Mu, J Qiao, L Qi et al. Appl. Mater. Interf., 2014, 6:21346-21354.
12. [12]
  X Q You, J P James. Sensor. Actuat. B, 2014, 202:1357-1365.
13. [13]
  K S Novoselov, A K Geim, S V Morozov et al. Science, 2004, 306:666-669.
14. [14]
  A K Geim, K S Novoselov. Nat. Mater., 2007, 6:183-191.
15. [15]
  Z G Yin, W W Zhao, M M Tian et al. Analyst, 2014, 139:1973-1979.
16. [16]
  B Liang, L Fang, G Yang et al. Biosens. Bioelectron., 2013, 43:131-136.
17. [17]
  Y P Li, Y F Bai, G Y Han et al. Sensor. Actuat. B., 2013, 185:706-712.
18. [18]
  P Onor, T Atul, A P F Turner et al. Biosens. Bioelectron., 2013, 49:53-62.
19. [19]
  D D Pan, Y Y Gua, H Z Lan. Anal. Chim. Acta, 2015, 853:297-302.
20. [20]
  J Yang, S Y Deng, J P Lei et al. Biosens. Bioelectron., 2011, 29:159-166.
21. [21]
  R S Dey, C R Raj. Biosens. Bioelectron., 2014, 62:357-364.
22. [22]
  H M Bao, Q W Chen, L Y Zhang. Analyst, 2011, 136:5190-5196.
23. [23]
  S R Cao, L Zhang, Y Q Chai et al. Talanta, 2013, 109:167-172.
24. [24]
  L M Lu, X L Qiu, X B Zhang et al. Biosens. Bioelectron., 2013, 45:102-107.
25. [25]
  T Y Yeh, C I Wang, H T Chang. Talanta, 2013, 115:718-723.
26. [26]
  B Jiang, K J Jang, Q Zhao. J. Chromatogr. A, 2012, 1254:8-13.
27. [27]
  G B Xu, X Y Chen, J H Hu et al. Analyst, 2012, 137:2757-2761.
28. [28]
  Q Zeng, J S Cheng X F Liu et al. Biosens. Bioelectron., 2011, 26:3456-3463.
29. [29]
  J Y Liu, E K Wang, J Wang et al. Electrochim. Acta. 2015, 161:17-22.
30. [30]
  Y Liu, Q Li, Y Y Feng et al. Chem. Lett., 2014, 68(6):732-738.
31. [31]
  M M Barsan, M David, M Florescu et al. Bioelectrochemistry, 2014, 99:46-52.
32. [32]
  W Wen, W Chen, Q Q Ren et al. Sensor Actuat. B, 2012, 166-167:444-450.
33. [33]
  L J Bai, B Yan, Y Q Chai et al. Analyst, 2013, 138:6595-6599.
34. [34]
  J Jiao, A Z Miao, X Y Zhang et al. Analyst, 2013, 138, 1645-1468.
35. [35]
  J Q Liu, N Kong, A H Li. Analyst, 2013, 138:2567-2575.
36. [36]
  D Zheng, S K Vashist, K A Rubeaan et al. Talanta, 2012, 99, 22-28.
37. [37]
  Q Zeng, J S Cheng, L H Tang et al. Adv. Funct. Mater., 2010, 20:3366-3372.
38. [38]
  Q L Sheng, M Z Wang, J B Zheng et al. Chin. J. Anal. Chem., 2009, 37(11):1557-1565.
39. [39]
  L Li, H Hu, L Deng. Talanta, 2013, 113:1-6.
40. [40]
  T Liu, H C Su, X J Qu et al. Sensor. Actuat. B., 2011, 160:1255-1261.
41. [41]
  L F Gao, H L Zhang, H Cui. Biosens. Bioelectron., 2014, 57:65-70.
1. [1]
  Chaolin Mi , Yuying Qin , Xinli Huang , Yijie Luo , Zhiwei Zhang , Chengxiang Wang , Yuanchang Shi , Longwei Yin , Rutao Wang . Galvanic Replacement Synthesis of Graphene Coupled Amorphous Antimony Nanoparticles for High-Performance Sodium-Ion Capacitor. Acta Physico-Chimica Sinica, 2024, 40(5): 2306011-0. doi: 10.3866/PKU.WHXB202306011
2. [2]
  Anbang Du , Yuanfan Wang , Zhihong Wei , Dongxu Zhang , Li Li , Weiqing Yang , Qianlu Sun , Lili Zhao , Weigao Xu , Yuxi Tian . Photothermal Microscopy of Graphene Flakes with Different Thicknesses. Acta Physico-Chimica Sinica, 2024, 40(5): 2304027-0. doi: 10.3866/PKU.WHXB202304027
3. [3]
  Zhihuan XU , Qing KANG , Yuzhen LONG , Qian YUAN , Cidong LIU , Xin LI , Genghuai TANG , Yuqing LIAO . Effect of graphene oxide concentration on the electrochemical properties of reduced graphene oxide/ZnS. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1329-1336. doi: 10.11862/CJIC.20230447
4. [4]
  Yuting Bai , Cenqi Yan , Zhen Li , Jiaqiang Qin , Pei Cheng . Preparation of High-Strength Polyimide Porous Films with Thermally Closed Pore Property by In Situ Pore Formation Method. Acta Physico-Chimica Sinica, 2024, 40(9): 2306010-0. doi: 10.3866/PKU.WHXB202306010
5. [5]
  Tao Xu , Wei Sun , Tianci Kong , Jie Zhou , Yitai Qian . Stable Graphite Interface for Potassium Ion Battery Achieving Ultralong Cycling Performance. Acta Physico-Chimica Sinica, 2024, 40(2): 2303021-0. doi: 10.3866/PKU.WHXB202303021
6. [6]
  Tiantian Zheng , Huiyi Wang , Huimin Li , Xuanhe Liu , Hong Shang . Anti-Counterfeiting National Salvation Chronicle of 006. University Chemistry, 2024, 39(9): 254-258. doi: 10.3866/PKU.DXHX202307032
7. [7]
  Wenli FENG , Lu ZHAO , Yunfeng BAI , Feng FENG . Research progress on ultralong room temperature phosphorescent carbon dots. Chinese Journal of Inorganic Chemistry, 2025, 41(5): 833-846. doi: 10.11862/CJIC.20240308
8. [8]
  Yuanyin Cui , Jinfeng Zhang , Hailiang Chu , Lixian Sun , Kai Dai . Rational Design of Bismuth Based Photocatalysts for Solar Energy Conversion. Acta Physico-Chimica Sinica, 2024, 40(12): 2405016-0. doi: 10.3866/PKU.WHXB202405016
9. [9]
  Miaomiao He , Zhiqing Ge , Qiang Zhou , Jiaqing He , Hong Gong , Lingling Li , Pingping Zhu , Wei Shao . Exploring the Fascinating Realm of Quantum Dots. University Chemistry, 2024, 39(6): 231-237. doi: 10.3866/PKU.DXHX202310040
10. [10]
  Laiying Zhang , Yaxian Zhu . Exploring the Silver Family. University Chemistry, 2024, 39(9): 1-4. doi: 10.12461/PKU.DXHX202409015
11. [11]
  Zhuo WANG , Junshan ZHANG , Shaoyan YANG , Lingyan ZHOU , Yedi LI , Yuanpei LAN . Preparation and photocatalytic performance of CeO₂-reduced graphene oxide by thermal decomposition. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1708-1718. doi: 10.11862/CJIC.20240067
12. [12]
  Hao BAI , Weizhi JI , Jinyan CHEN , Hongji LI , Mingji LI . Preparation of Cu₂O/Cu-vertical graphene microelectrode and detection of uric acid/electroencephalogram. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1309-1319. doi: 10.11862/CJIC.20240001
13. [13]
  Lisha LEI , Wei YONG , Yiting CHENG , Yibo WANG , Wenchao HUANG , Junhuan ZHAO , Zhongjie ZHAI , Yangbin DING . Application of regenerated cellulose and reduced graphene oxide film in synergistic power generation from moisture electricity generation and Mg-air batteries. Chinese Journal of Inorganic Chemistry, 2025, 41(6): 1151-1161. doi: 10.11862/CJIC.20240202
14. [14]
  Jie XIE , Hongnan XU , Jianfeng LIAO , Ruoyu CHEN , Lin SUN , Zhong JIN . Nitrogen-doped 3D graphene-carbon nanotube network for efficient lithium storage. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1840-1849. doi: 10.11862/CJIC.20240216
15. [15]
  Tian TIAN , Meng ZHOU , Jiale WEI , Yize LIU , Yifan MO , Yuhan YE , Wenzhi JIA , Bin HE . Ru-doped Co₃O₄/reduced graphene oxide: Preparation and electrocatalytic oxygen evolution property. Chinese Journal of Inorganic Chemistry, 2025, 41(2): 385-394. doi: 10.11862/CJIC.20240298
16. [16]
  Yunting Shang , Yue Dai , Jianxin Zhang , Nan Zhu , Yan Su . Something about RGO (Reduced Graphene Oxide). University Chemistry, 2024, 39(9): 273-278. doi: 10.3866/PKU.DXHX202306050
17. [17]
  Wei HE , Jing XI , Tianpei HE , Na CHEN , Quan YUAN . Application of solar-driven inorganic semiconductor-microbe hybrids in carbon dioxide fixation and biomanufacturing. Chinese Journal of Inorganic Chemistry, 2025, 41(1): 35-44. doi: 10.11862/CJIC.20240364
18. [18]
  Yihui Song , Shangshang Qin , Kai Wu , Chengyun Jin , Bin Yu . 生物化学在高水平创新型药学人才培养中的交叉融合应用——以去甲基化酶LSD1抑制剂的活性评价为例. University Chemistry, 2025, 40(6): 341-352. doi: 10.12461/PKU.DXHX202406018
19. [19]
  Xingyuan Lu , Yutao Yao , Junjing Gu , Peifeng Su . Energy Decomposition Analysis and Its Application in the Many-Body Effect of Water Clusters. University Chemistry, 2025, 40(3): 100-107. doi: 10.12461/PKU.DXHX202405074
20. [20]
  Yue-Zhou Zhu , Kun Wang , Shi-Sheng Zheng , Hong-Jia Wang , Jin-Chao Dong , Jian-Feng Li . Application and Development of Electrochemical Spectroscopy Methods. Acta Physico-Chimica Sinica, 2024, 40(3): 2304040-0. doi: 10.3866/PKU.WHXB202304040

Get Citation

PDF

XML

Metrics

PDF Downloads(43)
Abstract views(5616)
HTML views(2241)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142