Citation: Yang Shaoyuan, Zhu Jia, Wu Yan, Wang Xiaorui, Wu Haijing, Jia Zongchao, Zheng Jimin. Purification and Crystallization of MinC/FtsZ Complex in Escherichia coli[J]. Chemistry, ;2017, 80(1): 89-94. shu

Purification and Crystallization of MinC/FtsZ Complex in Escherichia coli

Collge of Chemistry, Beijing Normal University, Beijing 100875

Corresponding author: Zheng Jimin, jimin_z@bnu.edu.cn
Received Date: 12 September 2016
Accepted Date: 14 October 2016

Figures(4)

In Escherichia coli, cell division occurs when Z-ring forms at the mid-site of cell through polymerization of FtsZ. The Z-ring formation is inhibited by MinC, resulting in regulation of cell division. Herein, minC and ftsZ were cloned into vectors containing different antibiotics to express recombinant proteins, then MinC and FtsZ were purified by affinity chromatography and gel filtration chromatography. Two different methods, binding MinC and FtsZ after being expressed and purified respectively, and co-transformation, co-expression and co-purification of MinC and FtsZ were tried to gain MinC/FtsZ complex for crystal screen. Results showed that with appropriate conditions, through the way of binding MinC and FtsZ after being expressed and purified respectively, MinC/FtsZ complex were in the proportion of 1:1. The addition of GTP and MgCl₂ helped improve the protein state of MinC/FtsZ complex, and needle shape of MinC/FtsZ complex was observed after crystal screen. This study provides experimental basis for structural analysis of MinC/FtsZ complex.
- Cell division,
- Complex,
- Crystallization,
- MinC,
- FtsZ
1. [1]
  Y L Shih, L Rothfield. Microbiol. Mol. Biol. Rev., 2006, 70(3):729-754.
2. [2]
  E Bi, J Lutkenhaus. Nature, 1991, 354(6349):161-164.
3. [3]
  J Lowe, L A Amos. Nature, 1998, 391(6663):203-206.
4. [4]
  D Scheffers, A J Driessen. FEBS Lett., 2001, 506(1):6-10.
5. [5]
6. [6]
7. [7]
8. [8]
9. [9]
10. [10]
  Z Hu, J Lutkenhaus. Mol. Microbiol., 1999, 34(1):82-90.
11. [11]
  Z Hu, J Lutkenhaus. Mol. Cell, 2001, 7(6):1337-1343.
12. [12]
  B Shen, J Lutkenhaus. Mol. Microbiol., 2010, 75(5):1285-1298.
13. [13]
  W Wu, K T Park, T Holyoak et al. Mol. Microbiol., 2011, 79(6):1515-1528.
14. [14]
  A Dajkovic, G Lan, S X Sun et al. Curr. Biol., 2008, 18(4):235-244.
15. [15]
  S C Cordell, R E Anderson, J Lowe. EMBO J., 2001, 20(10):2454-2461.
16. [16]
  Z Hu, A Mukherjee, S Pichoff et al. PNAS, 1999, 96(26):14819-14824.
17. [17]
  P De Boer, R Crossley, L Rothfield. Nature, 1992, 359(6392):254-256.
18. [18]
  F Martin-Garcia, E Salvarelli, J I Mendieta-Moreno et al. FEBS Lett., 2012, 586(8):1236-1239.
19. [19]
  E Salvarelli, M Krupka, G Rivas et al. FEBS Lett., 2011, 585(24):3880-3883.
20. [20]
21. [21]
22. [22]
  P C Peters, M D Migocki, C Thoni et al. Mol. Microbiol., 2007, 64(2):487-499.
23. [23]
  D Shiomi, W Margolin. J. Bacteriol, 2007, 189(1):236-243.
24. [24]
  N R Stokes, J Sievers, S Barker et al. J. Biol. Chem., 2005, 280(48):39709-39715.
25. [25]
1. [1]
  Yan Xiao , Shuling Li , Yifan Li , Jianing Fan , Linlin Shi . Discovering the Beauty of Life: Adding Some “Ingredients” to Crystals. University Chemistry, 2024, 39(6): 366-372. doi: 10.3866/PKU.DXHX202312025
2. [2]
  Lanjun Cheng , Xinyuan Wang , Jie An , Xiang Wu , Chengfeng Zhu , Yanming Fu , Yougui Li . Improvement of the Resolution Experiment of Racemic Tartaric Acid. University Chemistry, 2025, 40(7): 277-285. doi: 10.12461/PKU.DXHX202408010
3. [3]
  Yongjie ZHANG , Bintong HUANG , Yueming ZHAI . Research progress of formation mechanism and characterization techniques of protein corona on the surface of nanoparticles. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2318-2334. doi: 10.11862/CJIC.20240247
4. [4]
  Xinran Zhang , Siqi Liu , Yichi Chen , Qingli Zou , Qinghong Xu , Yaqin Huang . From Protein to Energy Storage Materials: Edible Gelatin Jelly Electrolyte. University Chemistry, 2025, 40(7): 255-266. doi: 10.12461/PKU.DXHX202408104
5. [5]
  Zeyu XU , Anlei DANG , Bihua DENG , Xiaoxin ZUO , Yu LU , Ping YANG , Wenzhu YIN . Evaluation of the efficacy of graphene oxide quantum dots as an ovalbumin delivery platform and adjuvant for immune enhancement. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1065-1078. doi: 10.11862/CJIC.20240099
6. [6]
  Xinyi Hong , Tailing Xue , Zhou Xu , Enrong Xie , Mingkai Wu , Qingqing Wang , Lina Wu . Non-Site-Specific Fluorescent Labeling of Proteins as a Chemical Biology Experiment. University Chemistry, 2024, 39(4): 351-360. doi: 10.3866/PKU.DXHX202310010
7. [7]
  Ruolin CHENG , Yue WANG , Xiyao NIU , Huagen LIANG , Ling LIU , Shijian LU . Efficient photothermal catalytic CO₂ cycloaddition over W₁₈O₄₉/rGO composites. Chinese Journal of Inorganic Chemistry, 2025, 41(7): 1276-1284. doi: 10.11862/CJIC.20240424
8. [8]
  Jinfeng Chu , Lan Jin , Yu-Fei Song . Exploration and Practice of Flipped Classroom in Inorganic Chemistry Experiment: a Case Study on the Preparation of Inorganic Crystalline Compounds. University Chemistry, 2024, 39(2): 248-254. doi: 10.3866/PKU.DXHX202308016
9. [9]
  Huafeng SHI . Construction of MnCoNi layered double hydroxide@Co-Ni-S amorphous hollow polyhedron composite with excellent electrocatalytic oxygen evolution performance. Chinese Journal of Inorganic Chemistry, 2025, 41(7): 1380-1386. doi: 10.11862/CJIC.20240378
10. [10]
  Xuewei Qian , Xingwen Sun , Houjin Li , Zhanxiang Liu , Yuan Zheng , Lin Wu , Shuanglian Cai , Ying Xiong , Guangao Yu , Qingwen Liu , Jie Han , Xin Du , Chengshan Yuan , Qihan Zhang , Shuyong Zhang , Jianrong Zhang . Basic Operations and Specification Suggestions for Organic Chemical Recrystallization Experiments. University Chemistry, 2025, 40(5): 66-75. doi: 10.12461/PKU.DXHX202503126
11. [11]
  Chengshan Yuan , Xiaolong Li , Xiuping Yang , Xiangfeng Shao , Zitong Liu , Xiaolei Wang , Yongwen Shen . Standardized Operational Guidelines for Mixed-Solvent Recrystallization in Organic Chemistry Experiment. University Chemistry, 2025, 40(5): 122-127. doi: 10.12461/PKU.DXHX202504073
12. [12]
  Shi-Yu Lu , Wenzhao Dou , Jun Zhang , Ling Wang , Chunjie Wu , Huan Yi , Rong Wang , Meng Jin . Amorphous-Crystalline Interfaces Coupling of CrS/CoS₂ Few-Layer Heterojunction with Optimized Crystallinity Boosted for Water-Splitting and Methanol-Assisted Energy-Saving Hydrogen Production. Acta Physico-Chimica Sinica, 2024, 40(8): 2308024-0. doi: 10.3866/PKU.WHXB202308024
13. [13]
  Wei Zhong , Dan Zheng , Yuanxin Ou , Aiyun Meng , Yaorong Su . Simultaneously Improving Inter-Plane Crystallization and Incorporating K Atoms in g-C₃N₄ Photocatalyst for Highly-Efficient H₂O₂ Photosynthesis. Acta Physico-Chimica Sinica, 2024, 40(11): 2406005-0. doi: 10.3866/PKU.WHXB202406005
14. [14]
  Heng Chen , Longhui Nie , Kai Xu , Yiqiong Yang , Caihong Fang . Remarkable Photocatalytic H₂O₂ Production Efficiency over Ultrathin g-C₃N₄ Nanosheet with Large Surface Area and Enhanced Crystallinity by Two-Step Calcination. Acta Physico-Chimica Sinica, 2024, 40(11): 2406019-0. doi: 10.3866/PKU.WHXB202406019
15. [15]
  Chuanming GUO , Kaiyang ZHANG , Yun WU , Rui YAO , Qiang ZHAO , Jinping LI , Guang LIU . Performance of MnO₂-0.39IrO_x composite oxides for water oxidation reaction in acidic media. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1135-1142. doi: 10.11862/CJIC.20230459
16. [16]
  Ke Qiu , Fengmei Wang , Mochou Liao , Kerun Zhu , Jiawei Chen , Wei Zhang , Yongyao Xia , Xiaoli Dong , Fei Wang . A Fumed SiO₂-based Composite Hydrogel Polymer Electrolyte for Near-Neutral Zinc-Air Batteries. Acta Physico-Chimica Sinica, 2024, 40(3): 2304036-0. doi: 10.3866/PKU.WHXB202304036
17. [17]
  Zhaoxin LI , Ruibo WEI , Min ZHANG , Zefeng WANG , Jing ZHENG , Jianbo LIU . Advancements in the construction of inorganic protocells and their cell mimic and bio-catalytical applications. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2286-2302. doi: 10.11862/CJIC.20240235
18. [18]
  Yaping ZHANG , Tongchen WU , Yun ZHENG , Bizhou LIN . Z-scheme heterojunction β-Bi₂O₃ pillared CoAl layered double hydroxide nanohybrid: Fabrication and photocatalytic degradation property. Chinese Journal of Inorganic Chemistry, 2025, 41(3): 531-539. doi: 10.11862/CJIC.20240256
19. [19]
  Shuyu Liu , Xiaomin Sun , Bohan Song , Gaofeng Zeng , Bingbing Du , Chongshen Guo , Cong Wang , Lei Wang . Design and Fabrication of Phospholipid-Vesicle-based Artificial Cells towards Biomedical Applications. University Chemistry, 2024, 39(11): 182-188. doi: 10.12461/PKU.DXHX202404113
20. [20]
  Siyi ZHONG , Xiaowen LIN , Jiaxin LIU , Ruyi WANG , Tao LIANG , Zhengfeng DENG , Ao ZHONG , Cuiping HAN . Targeting imaging and detection of ovarian cancer cells based on fluorescent magnetic carbon dots. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1483-1490. doi: 10.11862/CJIC.20240093

Get Citation

PDF

XML

Metrics

PDF Downloads(10)
Abstract views(2737)
HTML views(699)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142