Citation: Kai ZHANG, Chao YAN, Zhi Chao ZHANG, Qin Sun WANG, Ru Yu GAO. Separation of Peptides by Pressurized Capillary Electrochromatography[J]. Chinese Chemical Letters, ;2003, 14(6): 611-614. shu

Separation of Peptides by Pressurized Capillary Electrochromatography

1.
State key Laboratory of Elemento-Organic Chemistry, Nankai University, Tianjin 300071;
2.
Unimicro Technologies, Inc., 4713 First Street, Pleasanton, CA 94566, USA

Received Date: 26 August 2002

Fund Project: The study is supported by NNSFC, grant No. 20175010.

A novel gradient pressurized capillary electrochromatography (pCEC) instrument wasdeveloped to separate peptides. Two gradient elution modes, hydrophobic and hydrophilicinteraction mode in pCEC, were performed on this instrument. Baseline separation of sixpeptides was obtained on two gradient modes with C18 column and strong cationic exchangecolumn respectively. The effects of mixer volume and total flow rate of pumps on resolutionwere also discussed.
- Pressurized capillary electrochromatography,
- peptide,
- separation,
- reversed phase,
- hydrophilic interaction
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