Citation: Tian Jiaqi, Chen Yuerong, Liu Ming, Zhang Yanhua, Sun Xudong, Fu Yao, Zhang Xiufeng, Shi Lei. A Cysteine Detection Probe Based on Regulating Cyanine Dye Transformation by G-quardruplex[J]. Chemistry, ;2020, 83(11): 1025-1030. shu

A Cysteine Detection Probe Based on Regulating Cyanine Dye Transformation by G-quardruplex

School of Chemical Engineering, North China University of Science and Technology, Tangshan, 063210

Corresponding author: Shi Lei, slbuct@sina.com
Received Date: 11 April 2020
Accepted Date: 17 July 2020

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Cysteine is a reducing amino acid that plays an important role in organisms, and changes of its content in the body may induce a variety of lesions. Therefore, cysteine detection technology with high selectivity, high sensitivity and low cost is of great significance. In this paper, a guanine-rich DNA sequence is induced by potassium and sodium metal ions to form a secondary structure that has a special response to mercury ion. The cyanine dye can recognize the DNA structure, and thus cause the transformation of the supramolecular aggregation, resulting in changes in its UV and visible spectral properties. Finally, the sensor composed of Hg²⁺ regulating G-quadruplex and cyanine dye is utilized in the highly selective detection of cysteine in the solution system.
- DNA G-quadruplex,
- Cyanine dyes,
- Thiol protein,
- Cysteine,
- UV spectrum
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