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Citation: Ma Xueyan, Wang Lin, Ding Lan, Zhao Daqing, Ni Jiazuan. Hydrophobic Pocket Modification and Glutathione Peroxidase Abzyme with High Activity Ⅱ.Synthesis and Characterization of Haptens and Antigens[J]. Chinese Journal of Applied Chemistry, ;1998, 15(1): 53-55. shu

Hydrophobic Pocket Modification and Glutathione Peroxidase Abzyme with High Activity Ⅱ.Synthesis and Characterization of Haptens and Antigens

Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022

Received Date: 25 March 1997
Revised Date: 14 August 1997

The thiol group of glutathione (GSH) was protected by 2, 4-dinitrochlorobenzene (DNCB). The product GSH-S-DNP was reacted with 2-methyl-1-propanol and 3-methyl-1-butanol respectively to obtain hapten 6 and 7. Their structures were determined by 400 MHz ¹H NMR and IR. The two haptens were then covalently crosslinked with bovine serum albu-min(BSA) hy using glutaraldehyde as coupling agent to obtain antigen 6 and 7. The results of gel electrophoresis analysis gave the average molecular weight of 1.27 × 10^-22 kg for each antigen. The average connected ratio of BSA to hapten was 1:12. Other UV and fluores-cence spectral properties of the antigens are reported.
- glutathione peroxidase,
- abzyme,
- hydrophobic hapten,
- preparation,
- hydrophobic pocket modification
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