Citation: Meng Erjuan, Sang Yanxiao, Zhu Zhihui, Liu Shilin, Hu Fangzhong, Ren Xueling. Specific Cleavage of Supercoiled Plasmid DNA by (E)-2-Cyano-3-[4-(2, 4-difluorophenoxy) benzylamino]-3-methoxyacrylic acid 2-Ethoxyethyl Ester[J]. Chemistry, ;2017, 80(10): 953-957. shu

Specific Cleavage of Supercoiled Plasmid DNA by (E)-2-Cyano-3-[4-(2, 4-difluorophenoxy) benzylamino]-3-methoxyacrylic acid 2-Ethoxyethyl Ester

1.
Key Laboratory of Targeting Therapy and Diagnosis for Critical Diseases, Henan, School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450001
2.
Institute of Elemento-Organic Chemistry, Nankai University, Tianjin 300071

Corresponding author: Ren Xueling, renxueling@zzu.edu.cn
Received Date: 29 March 2017
Accepted Date: 7 June 2017

Figures(5)

Here a novel cyanoacrylic ester, (E)-2-cyano-3-[4-(2, 4-difluorophenoxy) benzylamino]-3-methoxyacrylic acid 2-ethoxyethyl ester (MAE), which is capable of site-specific cleavage of supercoiled pUC19 DNA, was introduced.The cleavage specificity was investigated through UV absorption, fluorescence, agarose gel electrophoresis, and DNA sequencing techniques.Red shift and hypochromism in the absorption spectra and static fluorescence quenching suggested that a typical intercalation occurred between DNA and MAE.In addition, detectable varieties in the gel electrophoresis and DNA sequencing indicated that the cleavage was site-specific, and double strands of DNA were hydrolyzed step by step.
- DNA cleavage,
- Acrylic ester,
- Spectroscopy,
- DNA sequencing
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