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Citation: Zhu Zhenqi, Ding Lan, Luo Guimin, Sun Qi'an, Yang Tongshu, Shen Jiacong. Studies on Catalytic Antibody with Glutathione Peroxidase Activity Ⅰ.Synthesis and Property of Glutathione Derivatives as Specific Antigens[J]. Chinese Journal of Applied Chemistry, ;1994, 44(5): 87-90. shu

Studies on Catalytic Antibody with Glutathione Peroxidase Activity Ⅰ.Synthesis and Property of Glutathione Derivatives as Specific Antigens

1.
1. The National Laboratory of Enzyme Engineering, Jilin University, Changchun 130023;
2.
2. Key Laboratory of Molecular Spectrum and Molecular Structure, Jilin University, Changchun

Received Date: 27 October 1993
Available Online: 5 April 1994

The sulfhydryl groups of the reduced glutathione (GSH) have been reacted with2,4-dinitrochlorobenzene to give S-protective glutathione as a hapten,The haptens werecrosslinked with the amino groups on the surface of BSA (Bovine Serum Albumin) glutaraldehyde,The covalent conjugates are GSH specific antigens of high immunogenicity.The antigens were found to be homogenious and have a molecular weight of 87000 dalton asdetermined by gel-electrophoresis method.Spectral and circular dichroism measurementsshowed that the antigens possess special vis-UV spectra,fluorescence nature with aincreased compact of antigen structure.
- glutathione peroxidase,
- catalytic antibody,
- specific antigen,
- preparation
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